• Product name
    Anti-Cytokeratin 18 antibody [DA-7] (Biotin)
    See all Cytokeratin 18 primary antibodies
  • Description
    Mouse monoclonal [DA-7] to Cytokeratin 18 (Biotin)
  • Host species
  • Conjugation
  • Specificity
    ab27549 recognises Cytokeratin peptide 18. We have data to indicate that this antibody may not cross react with wide range of other species. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: WB, ELISA, ICC, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human breast carcinoma cell line PMC-42.

  • Positive control
    • Western blot and immunocytochemistry: HeLa human cervix carcinoma cell line, MCF-7 human breast adenocarcinoma cell line. Immunohistochemistry: epithelial tissue (skin). ELISA: MCF-7 human breast adenocarcinoma cell line



Our Abpromise guarantee covers the use of ab27549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 45 kDa.
ELISA Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
IP Use at an assay dependent dilution.


  • Function
    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificity
    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in disease
    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Post-translational
    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localization
    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all


  • Immunocytochemistry/Immunofluorescence analysis of human ovarian tumour tissue labelling Cytokeratin 18 with ab7549 conjugated to conjugated to QD655 at a molar ratio of 6:1 (Ab:QD). Tissue was incubated with primary antibody overnight at 4°C. Right image - tissue was incubated a further 2 hours at room temperature in order to remove background staining.

  • Anti-Cytokeratin 18 antibody [DA-7] (Biotin) (ab27549) at 1 µg/ml + HeLa (50 million) cells re-suspended in 1 ml cold Lysis buffer.

    Performed under non-reducing conditions.

    Predicted band size: 45 kDa


This product has been referenced in:
  • Sweeney E  et al. Quantitative multiplexed quantum dot immunohistochemistry. Biochem Biophys Res Commun 374:181-6 (2008). ICC ; Human . Read more (PubMed: 18621021) »
See 1 Publication for this product

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