Overview

  • Product name

    Anti-Cytokeratin 18 antibody [DC 10]
    See all Cytokeratin 18 primary antibodies
  • Description

    Mouse monoclonal [DC 10] to Cytokeratin 18
  • Host species

    Mouse
  • Specificity

    Specific reactivity for the cytokeratin 18 (45 kD) polypeptide.
  • Tested applications

    Suitable for: IHC-Fr, ICC, IHC-P, Flow Cyt, ICC/IF, ELISA, WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Sheep, Hamster, Cow, Dog, Pig, a wide range of other species
  • Immunogen

    PMC-42 human breast carcinoma cells.

  • Positive control

    • IHC-P: Colon carcinoma WB, ELISA, IP: MCF-7 or HeLa cell lysate
  • General notes

    This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from TCS
  • Primary antibody notes

    This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
  • Clonality

    Monoclonal
  • Clone number

    DC 10
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P 1/10 - 1/100.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100.
ELISA Use at an assay dependent concentration. (Direct)
WB 1/1000.
IP Use at an assay dependent concentration.

Target

  • Function

    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificity

    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in disease

    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localization

    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Images

  • Ab7797 staining cells from a human pancreatic cancer cell line by ICC/IF.  The cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C.  Ab7797 was diluted 1/100 and incubated with the sample for 1 hour at 22°C.  An Alexa Fluor® 488 conjugated donkey anti-mouse antibody was used as the secondary.

    See Abreview

  • Immunohistochemistry staining of human colon (paraffin sections) using ab7797 10 μg/ml

  • Anti-Cytokeratin 18 antibody [DC 10] (ab7797) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • ab7797 staining human LNCaP cells by ICC/IF.  Cells were acetone fixed and permeabilized in 0.1% Triton-X prior to blocking with 1% fish gelatin for 1 hour at 4°C.  The primary antibody was diluted 1/100 and incubated with the sample for 1 hour.  An Alexa Fluor® conjugated goat anti-mouse antibody, diluted 1/1000, was used as the secondary.

    See Abreview

  • Overlay histogram showing MCF-7 cells stained with ab7797 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7797, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight ® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

References

This product has been referenced in:

  • Al-Khalaf HH  et al. Senescent Breast Luminal Cells Promote Carcinogenesis through Interleukin-8-Dependent Activation of Stromal Fibroblasts. Mol Cell Biol 39:N/A (2019). Read more (PubMed: 30397077) »
  • Deng H  et al. Susceptibility of epithelial cells cultured from different regions of human cervix to HPV16-induced immortalization. PLoS One 13:e0199761 (2018). Read more (PubMed: 29944714) »
See all 11 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human retinal pigment epithelium (ARPE-19))
Permeabilization
Yes - TritonX 0,3%
Specification
Human retinal pigment epithelium (ARPE-19)
Blocking step
Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Methanol

Abcam user community

Verified customer

Submitted Jun 26 2015

Application
ELISpot
Sample
Human Recombinant protein (E coli)
Specification
E coli

Abcam user community

Verified customer

Submitted May 12 2015

Application
ELISA
Sample
Human Recombinant protein (Human Cytokeratin 18 full length protein (ab73638))
Specification
Human Cytokeratin 18 full length protein (ab73638)
Type
Sandwich (Detection)
Blocking step
StartingBlock blocking buffer (Thermo Scientific) as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 100% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Feb 25 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Human Cell (hIPSC-derived endocrine cells)
Specification
hIPSC-derived endocrine cells
Permeabilization
Yes - 0.1 % triton x100
Fixative
Formaldehyde

Dr. Youngjin Kim

Verified customer

Submitted Mar 24 2014

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human breast carcinoma xenograft in mouse)
Specification
Human breast carcinoma xenograft in mouse
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Perkin-Elmer TNB Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 01 2009

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MDA468)
Specification
MDA468
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 01 2009

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (C4-2b (prostate cancer cell line))
Specification
C4-2b (prostate cancer cell line)
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 0.2% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jul 15 2008

Application
Western blot
Sample
Human Cell lysate - whole cell (pancreatic cancer cell line)
Loading amount
20 µg
Specification
pancreatic cancer cell line
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Hannah Whiteman

Verified customer

Submitted Jul 04 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (pancreatic cancer cell line)
Specification
pancreatic cancer cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C

Dr. Hannah Whiteman

Verified customer

Submitted Jun 18 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP)
Specification
LNCaP
Fixative
Acetone
Permeabilization
Yes - 0.1% Triton-X
Blocking step
Fish Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Dr. Vivien D'Arcy

Verified customer

Submitted Mar 06 2008

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