Overview

  • Product name

    Anti-Cytokeratin 18 antibody [DC10]
    See all Cytokeratin 18 primary antibodies
  • Description

    Mouse monoclonal [DC10] to Cytokeratin 18
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Cytokeratin 18. Human breast cancer PMC42 cells.

  • Positive control

    • IHC-P: Human breast carcinoma, breast, ductal carcinoma, hepatocellular carcinoma, ductal carcinoma, prostate, prostate adenocarcinoma, colon, colon adenocarcinoma, bladder, bladder transitional cell carcinoma and liver tissues. WB: A431 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab231431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Boil tissue section in Citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Incubate with primary antibody for 30 minutes at room temperature.

WB 1/25. Predicted molecular weight: 48 kDa.

Target

  • Function

    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificity

    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in disease

    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localization

    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Images

  • Anti-Cytokeratin 18 antibody [DC10] (ab231431) at 1/25 dilution + A431 (human epidermoid carcinoma cell line) cell lysate.

    Developed using the ECL technique.

    Predicted band size: 48 kDa

  • Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human breast tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human prostate tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human prostate Adenocarcinoma tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon Adenocarcinoma tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human bladder tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human liver tissue stained for Cytokeratin 18 using ab231431 at 1/100 dilution in immunohistochemical analysis.

References

ab231431 has not yet been referenced specifically in any publications.

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