Recombinant
RabMAb

Recombinant Anti-Cytokeratin 18 antibody [EPR1611] (ab133302)

Overview

  • Product name

    Anti-Cytokeratin 18 antibody [EPR1611]
    See all Cytokeratin 18 primary antibodies
  • Description

    Rabbit monoclonal [EPR1611] to Cytokeratin 18
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Cytokeratin 18 (P05783).

  • Positive control

    • MCF-7, A431, and HeLa whole cell lysate (ab150035), HeLa cells
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 48 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
    • Tissue specificity

      Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
    • Involvement in disease

      Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
    • Sequence similarities

      Belongs to the intermediate filament family.
    • Post-translational
      modifications

      Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
      Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
      O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
    • Cellular localization

      Cytoplasm > perinuclear region.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cell proliferation inducing gene 46 protein antibody
      • Cell proliferation inducing protein 46 antibody
      • Cell proliferation-inducing gene 46 protein antibody
      • CK 18 antibody
      • CK-18 antibody
      • CK18 antibody
      • CYK 18 antibody
      • CYK18 antibody
      • Cytokeratin 18 antibody
      • Cytokeratin endo B antibody
      • Cytokeratin-18 antibody
      • K 18 antibody
      • K18 antibody
      • K1C18_HUMAN antibody
      • KA18 antibody
      • Keratin 18 antibody
      • Keratin 18, type I antibody
      • Keratin D antibody
      • keratin, type I cytoskeletal 18 antibody
      • Keratin-18 antibody
      • Krt18 antibody
      see all

    Images

    • All lanes : Anti-Cytokeratin 18 antibody [EPR1611] (ab133302) at 1/10000 dilution

      Lane 1 : MCF-7 cell lysates
      Lane 2 : A431 cell lysates
      Lane 3 : HeLa cell lysates

      Predicted band size: 48 kDa

    • Immunofluorescence analysis of HeLa cells labelled with ab133302 at 1/100 dilution.

    References

    This product has been referenced in:

    • Jeong BY  et al. Oxidative stress caused by activation of NADPH oxidase 4 promotes contrast-induced acute kidney injury. PLoS One 13:e0191034 (2018). Read more (PubMed: 29329317) »
    • Hirai A  et al. Prognostic impact of programmed death-ligand 1 expression in correlation with human leukocyte antigen class I expression status in stage I adenocarcinoma of the lung. J Thorac Cardiovasc Surg 155:382-392.e1 (2018). Read more (PubMed: 28711324) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Answer

    Although mouse samples were not recommended based on the western blot results below with these antibodies, there is still the possibility that the negative results in mouse might be due to the limited sample types, especially for the antibodies with high homology (more than 80%) with mouse proteins.
    CK 18
    ab32118: waiting homology and mouse testing
    CK 8
    ab32357: waiting homology, blank WB in mouse brain, heart, lung, muscle, spleen
    CK 7
    ab52870: no homology, no mouse testing performed due to no homology
    CK 19
    ab76539: 80% homology, blank WB in mouse brain, heart, kidney, spleen
    CK 18
    ab133272: 93% homology, blank WB in mouse brain, heart, kidney, spleen, NIH/3T3, Raw264.7
    CK 18
    ab133302: 86% homology, blank WB in mouse brain, heart, kidney, spleen, NIH/3T3, Raw 264.7
    Vimentin
    ab133260: 100% homology, blank WB in mouse brain, heart, kidney, spleen. Non-specific bands in NIH/3T3.
    Epcam
    I could not find any antibodies in our catalog that have low homology with mouse. Your best option is to look for an antibody with an immunogen between human Epcam aa 36-53, the least homologous region of the protein with mouse Epcam. We do provide custom antibody creation in which we can create a rabbit monoclonal antibody with a synthetic peptide in a region of your choosing, please let me know if this is something you are interested in, and I'd be happy to put you in contact with our custom antibody services group.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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