Recombinant Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)

Thank you for your response.

It is important to mention that only ab133263 has been tested for IHC on formalin-fixed tissue samples. The other product (ab82254)is suitable for frozen sections, not been tested for formalin-fixed tissue samples.

I hope this helps and if I can assist further, please do not hesitate to contact me.

Thank you for your enquiry and your interest in our products.

Here are a few useful and interesting websites, you may wish to read through before starting the experiments:
https://docs.abcam.com/pdf/immunology/fluorochromes_table_updated.pdf
https://www.abcam.com/index.html?pageconfig=resource&rid=12922
https://www.abcam.com/index.html?pageconfig=popular_protocols
Both antibodies are suitable for detecting rat cytokeratin 18 and have been tested for immunohistochemistry. You may need to consider how you wish to fix the samples i.e. formalin, acetone, methanol, ethanol etc.









These antibodies are unconjugated, so you need a compatible secondary antibody which is conjugated for the detection. In order to be able to select the right secondary antibody, it is essential to understand the isotype and the host species of your chosen primary antibody:

- ab82254: mouse IgG1 so the secondary should be anti-mouse IgG1;

- ab133263: rabbit IgG1 so the secondary should be anti-rabbit IgG1;

I wouldrecommend conducting a search for the secondary antibody using the advance search engine. You can select the target species, the host species, isotype, format, conjugate etc:

https://www.abcam.com/index.html?pageconfig=productmap&cl=918

I hope this helps and if I can assist further, please do not hesitate to contact me.