Recombinant
RabMAb

Recombinant Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)

Overview

  • Product name

    Anti-Cytokeratin 18 antibody [EPR1626]
    See all Cytokeratin 18 primary antibodies
  • Description

    Rabbit monoclonal [EPR1626] to Cytokeratin 18
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, Flow Cyt, WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region within the C-terminal end of Human Cytokeratin 18 (P05783).

  • Positive control

    • MCF7 cell lysate, HeLa whole cell lysate (ab150035), Jurkat whole cell lysate (ab7899), A431 cell lysate, Human gastric adenocarcinoma tissue, Human breast tissue, HeLa cells.
  • General notes

     

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent concentration.
WB 1/10000 - 1/50000. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).
IHC-P 1/250 - 1/500. Perform antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
    • Tissue specificity

      Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
    • Involvement in disease

      Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
    • Sequence similarities

      Belongs to the intermediate filament family.
    • Post-translational
      modifications

      Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
      Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
      O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
    • Cellular localization

      Cytoplasm > perinuclear region.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cell proliferation inducing gene 46 protein antibody
      • Cell proliferation inducing protein 46 antibody
      • Cell proliferation-inducing gene 46 protein antibody
      • CK 18 antibody
      • CK-18 antibody
      • CK18 antibody
      • CYK 18 antibody
      • CYK18 antibody
      • Cytokeratin 18 antibody
      • Cytokeratin endo B antibody
      • Cytokeratin-18 antibody
      • K 18 antibody
      • K18 antibody
      • K1C18_HUMAN antibody
      • KA18 antibody
      • Keratin 18 antibody
      • Keratin 18, type I antibody
      • Keratin D antibody
      • keratin, type I cytoskeletal 18 antibody
      • Keratin-18 antibody
      • Krt18 antibody
      see all

    Images

    • Immunofluorescence analysis of HeLa cells labelling Cytokeratin 18 with ab133263 at 1/250.
    • Immunohistochemical analysis of paraffin embedded Human gastric adenocarcinoma tissue labelling Cytokeratin 18 with ab133263 at 1/250.

    • All lanes : Anti-Cytokeratin 18 antibody [EPR1626] (ab133263) at 1/10000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 48 kDa

    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Cytokeratin 18 with unpurified ab133263 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 80% methanol and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Immunohistochemical analysis of paraffin embedded Human breast tissue labelling Cytokeratin 18 with ab133263 at 1/250.
    • ab133263 showing positive staining in Prostatic carcinoma tissue.

    • ab133263 showing negative staining in Skeletal muscle tissue.

    • ab133263 showing negative staining in Normal brain tissue.

    • ab133263 showing positive staining in Breast carcinoma tissue.

    • ab133263 showing positive staining in Lung adenocarcinoma tissue.

    • ab133263 showing negative staining in Cervical squamous carcinoma tissue.

    References

    This product has been referenced in:

    • Chen X  et al. Increase of glandular epithelial cell clusters by an external volume expansion device promotes adipose tissue regeneration by recruiting macrophages. Biosci Rep 39:N/A (2019). Read more (PubMed: 30760630) »
    • Allen H  et al. Human Placental-Derived Adherent Stromal Cells Co-Induced with TNF-a and IFN-? Inhibit Triple-Negative Breast Cancer in Nude Mouse Xenograft Models. Sci Rep 8:670 (2018). Read more (PubMed: 29330447) »
    See all 9 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% TritonX100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Apr 22 2015

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Sample
    Human Tissue sections (A549 cell line)
    Specification
    A549 cell line
    Permeabilization
    Yes - 0.5% Triton X100
    Fixative
    Paraformaldehyde

    Mrs. Aleksandra Kotlyarova

    Verified customer

    Submitted Sep 26 2013

    Answer

    Thank you for your response.

    It is important to mention that only ab133263 has been tested for IHC on formalin-fixed tissue samples. The other product (ab82254)is suitable for frozen sections, not been tested for formalin-fixed tissue samples.

    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    Answer

    Thank you for your enquiry and your interest in our products.

    Here are a few useful and interesting websites, you may wish to read through before starting the experiments:
    https://docs.abcam.com/pdf/immunology/fluorochromes_table_updated.pdf
    https://www.abcam.com/index.html?pageconfig=resource&rid=12922
    https://www.abcam.com/index.html?pageconfig=popular_protocols
    Both antibodies are suitable for detecting rat cytokeratin 18 and have been tested for immunohistochemistry. You may need to consider how you wish to fix the samples i.e. formalin, acetone, methanol, ethanol etc.









    These antibodies are unconjugated, so you need a compatible secondary antibody which is conjugated for the detection. In order to be able to select the right secondary antibody, it is essential to understand the isotype and the host species of your chosen primary antibody:

    - ab82254: mouse IgG1 so the secondary should be anti-mouse IgG1;

    - ab133263: rabbit IgG1 so the secondary should be anti-rabbit IgG1;

    I wouldrecommend conducting a search for the secondary antibody using the advance search engine. You can select the target species, the host species, isotype, format, conjugate etc:

    https://www.abcam.com/index.html?pageconfig=productmap&cl=918

    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

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