Name: Anti-Cytokeratin 18 antibody [EPR1626]
Brand: RabMAb
SKU: ab133263
Rating: 5 (2 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1626] to Cytokeratin 18
Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
Reacts with: Rat, Human

Product name

Anti-Cytokeratin 18 antibody [EPR1626]
See all Cytokeratin 18 primary antibodies
Description

Rabbit monoclonal [EPR1626] to Cytokeratin 18
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
Unsuitable for: IP
Species reactivity

Reacts with: Rat, Human
Immunogen

Synthetic peptide corresponding to a region within the C-terminal end of Human Cytokeratin 18 (P05783).
Positive control
- WB: HeLa, MCF7, and Rat liver, HeLa whole cell lysate (ab150035), Jurkat whole cell lysate (ab7899), and A431 cell lysates. IHC-P: human endometrium, Human gastric adenocarcinoma tissue, Human breast tissue, and rat liver tissues. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR1626
Isotype

IgG
Research areas

Alternative Versions
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Jurkat cytoplasmic extract lysate (ab14842)

Our Abpromise guarantee covers the use of ab133263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt		1/20.
WB		1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa). For unpurified use at 1/10,000 - 1/50,000
IHC-P		1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. 1:500 in human tissues / 1:1000 in rat tissues
ICC/IF		1/50. For unpurified use at 1/250 - 1/500

Application notes

Is unsuitable for IP.

Function

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Tissue specificity

Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
Involvement in disease

Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
Sequence similarities

Belongs to the intermediate filament family.
Post-translational
modifications

Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
Cellular localization

Cytoplasm > perinuclear region.
Target information above from: UniProt accession P05783 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 3875 Human
- Entrez Gene: 294853 Rat
- Omim: 148070 Human
- SwissProt: P05783 Human
- SwissProt: Q5BJY9 Rat
- Unigene: 406013 Human
- Unigene: 103924 Rat
Alternative names
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
- Cell proliferation-inducing gene 46 protein antibody
- CK 18 antibody
- CK-18 antibody
- CK18 antibody
- CYK 18 antibody
- CYK18 antibody
- Cytokeratin 18 antibody
- Cytokeratin endo B antibody
- Cytokeratin-18 antibody
- K 18 antibody
- K18 antibody
- K1C18_HUMAN antibody
- KA18 antibody
- Keratin 18 antibody
- Keratin 18, type I antibody
- Keratin D antibody
- keratin, type I cytoskeletal 18 antibody
- Keratin-18 antibody
- Krt18 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue sections labeling Cytokeratin 18 with Purified ab133263 at 1:500 dilution (0.23 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Flow Cytometry - Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytokeratin 18 with Purified ab133263 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)

All lanes : Anti-Cytokeratin 18 antibody [EPR1626] (ab133263) at 1/20000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 45 kDa
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytokeratin 18 with Purified ab133263 at 1:50 dilution (2.26 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Alexa Fluor^® 488 (ab206091) and Alexa Fluor^® 647 (ab206269) conjugated versions are available for this clone.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling Cytokeratin 18 with Purified ab133263 at 1:1000 dilution (0.113 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)

Anti-Cytokeratin 18 antibody [EPR1626] (ab133263) at 1/1000 dilution (Purified) + Rat liver lysates at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution

Predicted band size: 48 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)

Immunofluorescence analysis of HeLa cells labelling Cytokeratin 18 with ab133263 at 1/250.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)

Immunohistochemical analysis of paraffin embedded Human gastric adenocarcinoma tissue labelling Cytokeratin 18 with ab133263 at 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)

All lanes : Anti-Cytokeratin 18 antibody [EPR1626] (ab133263) at 1/10000 dilution

Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A431 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 48 kDa
Flow Cytometry - Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)

Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Cytokeratin 18 with unpurified ab133263 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 80% methanol and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor^® 488)(ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)

Immunohistochemical analysis of paraffin embedded Human breast tissue labelling Cytokeratin 18 with ab133263 at 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)

ab133263 showing positive staining in Prostatic carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)

ab133263 showing negative staining in Skeletal muscle tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)

ab133263 showing negative staining in Normal brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)

ab133263 showing positive staining in Breast carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)

ab133263 showing positive staining in Lung adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)

ab133263 showing negative staining in Cervical squamous carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet

Publishing research using ab133263? Please let us know so that we can cite the reference in this datasheet.

ab133263 has been referenced in 14 publications.

Liu G et al. IKKe phosphorylates kindlin-2 to induce invadopodia formation and promote colorectal cancer metastasis. Theranostics 10:2358-2373 (2020). PubMed: 32104508
Ahn YH et al. A three-dimensional hyaluronic acid-based niche enhances the therapeutic efficacy of human natural killer cell-based cancer immunotherapy. Biomaterials 247:119960 (2020). PubMed: 32278822
Chen YL et al. Novel circulating tumor cell-based blood test for the assessment of PD-L1 protein expression in treatment-naïve, newly diagnosed patients with non-small cell lung cancer. Cancer Immunol Immunother 68:1087-1094 (2019). PubMed: 31089757
Zhang J et al. KRT18 is correlated with the malignant status and acts as an oncogene in colorectal cancer. Biosci Rep 39:N/A (2019). PubMed: 31345960
Chen X et al. Increase of glandular epithelial cell clusters by an external volume expansion device promotes adipose tissue regeneration by recruiting macrophages. Biosci Rep 39:N/A (2019). PubMed: 30760630

View all Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HeLa)

Permeabilization

Yes - 0.5% TritonX100 in PBS

Specification

HeLa

Fixative

Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Apr 22 2015

Application

Immunohistochemistry (Frozen sections)

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Sample

Human Tissue sections (A549 cell line)

Specification

A549 cell line

Permeabilization

Yes - 0.5% Triton X100

Fixative

Paraformaldehyde

Mrs. Aleksandra Kotlyarova

Verified customer

Submitted Sep 26 2013

Question

Thanks for your replay and all the useful information
I have already fixed all the tissue with formalin 4% … is it possible to
use the antibody for fixed tissue?
Thanks

Abcam community

Verified customer

Asked on Jun 01 2012

Answer

Thank you for your response.

It is important to mention that only ab133263 has been tested for IHC on formalin-fixed tissue samples. The other product (ab82254)is suitable for frozen sections, not been tested for formalin-fixed tissue samples.

I hope this helps and if I can assist further, please do not hesitate to contact me.

Abcam Scientific Support

Answered on Jun 01 2012

Question

Inquiry: Dear Sir or Madam I want to stain a fixed rat mammary gland tissue with fluorescent dye have excitation and emission wave length between 632/650 I thought (Anti-Cytokeratin 18 antibody [CK-18] (ab82254)) or (Anti-Cytokeratin 18 antibody [EPR1626] (ab133263)) could be right choice for my purpose? Could you please confirm to me if I am right or if there is better choice could you please recommend to me? I am microbiologist and my background in histology is not very good … that is why I need a complete product to use directly for the specific stain (the antibody with the fluorescent stain together) is it available such product with the condition I request above (fixed rat mammary tissue at Ex/Em 632/650)? My last aim is to get a red fluorescent photo for the tissue like the ones you present in the file of the two items ((ab133263) and (ab82254)) please send to me your answer as soon as possible? Many thanks in advance

Abcam community

Verified customer

Asked on May 31 2012

Answer

Thank you for your enquiry and your interest in our products.

Here are a few useful and interesting websites, you may wish to read through before starting the experiments:
https://docs.abcam.com/pdf/immunology/fluorochromes_table_updated.pdf
https://www.abcam.com/index.html?pageconfig=resource&rid=12922
https://www.abcam.com/index.html?pageconfig=popular_protocols
Both antibodies are suitable for detecting rat cytokeratin 18 and have been tested for immunohistochemistry. You may need to consider how you wish to fix the samples i.e. formalin, acetone, methanol, ethanol etc.

These antibodies are unconjugated, so you need a compatible secondary antibody which is conjugated for the detection. In order to be able to select the right secondary antibody, it is essential to understand the isotype and the host species of your chosen primary antibody:

- ab82254: mouse IgG1 so the secondary should be anti-mouse IgG1;

- ab133263: rabbit IgG1 so the secondary should be anti-rabbit IgG1;

I wouldrecommend conducting a search for the secondary antibody using the advance search engine. You can select the target species, the host species, isotype, format, conjugate etc:

https://www.abcam.com/index.html?pageconfig=productmap&cl=918

I hope this helps and if I can assist further, please do not hesitate to contact me.

Abcam Scientific Support

Answered on May 31 2012

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For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Applications

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References (14)

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 18 antibody [EPR1626]

Immunohistochemistry (Frozen sections) abreview for Anti-Cytokeratin 18 antibody [EPR1626]

Post-translational
modifications