Key features and details
- Mouse monoclonal [KRT18/1190] to Cytokeratin 18
- Suitable for: IHC-P
- Reacts with: Rat, Human
- Isotype: IgG1
Product nameAnti-Cytokeratin 18 antibody [KRT18/1190]
See all Cytokeratin 18 primary antibodies
DescriptionMouse monoclonal [KRT18/1190] to Cytokeratin 18
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Rat, Human
Recombinant full length protein corresponding to Human Cytokeratin 18 aa 2-430.
SFTTRSTFSTNYRSLGSVQAPSYGARPVSSAASVYAGAGGSGSRISVSRS TSFRGGMGSGGLATGIAGGLAGMGGIQNEKETMQSLNDRLASYLDRVRSL ETENRRLESKIREHLEKKGPQVRDWSHYFKIIEDLRAQIFANTVDNARIV LQIDNARLAADDFRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLET EIEALKEELLFMKKNHEEEVKGLQAQIASSGLTVEVDAPKSQDLAKIMAD IRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRR TVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQ TRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNS MQTIQKTTTRRIVDGKVVSETNDTKVLRH
Database link: P05783
- IHC-P: Human prostate carcinoma and rat colon tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from Bioreactor Concentrate by Protein A/G.
Light chain typekappa
Our Abpromise guarantee covers the use of ab233913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Primary incubation for 30 minutes at room temperature.
FunctionInvolved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Tissue specificityExpressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
Involvement in diseaseDefects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
Sequence similaritiesBelongs to the intermediate filament family.
modificationsPhosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
Cellular localizationCytoplasm > perinuclear region.
- Information by UniProt
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233913 has not yet been referenced specifically in any publications.