Anti-Cytokeratin 18 antibody [KRT18/1190] - BSA and Azide free (ab212555)

Overview

  • Product name

    Anti-Cytokeratin 18 antibody [KRT18/1190] - BSA and Azide free
    See all Cytokeratin 18 primary antibodies
  • Description

    Mouse monoclonal [KRT18/1190] to Cytokeratin 18 - BSA and Azide free
  • Host species

    Mouse
  • Specificity

    ab212555 reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein.
    Database link: P05783

  • Positive control

    • Human prostate carcinoma and breast cancer tissues; Rat colon tissue; MCF7 and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab212555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 2 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.

(in 0.1 ml).

IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

(Prmary incubation for 30 min at RT).

Target

  • Function

    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificity

    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in disease

    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localization

    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labeling Cytokeratin 18 with ab212555 at 1 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat colon tissue labeling Cytokeratin 18 with ab212555 at 1 µg/ml.

References

ab212555 has not yet been referenced specifically in any publications.

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