Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)

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Overview

  • Product name

    Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190]
    See all Cytokeratin 18 primary antibodies

  • Description

    Mouse monoclonal [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] to Cytokeratin 18

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Recombinant full length protein corresponding to Human Cytokeratin 18 aa 1-430.
    Sequence:

    MSFTTRSTFS TNYRSLGSVQ APSYGARPVS SAASVYAGAG GSGSRISVSR STSFRGGMGS GGLATGIAGG LAGMGGIQNE KETMQSLNDR LASYLDRVRS LETENRRLES KIREHLEKKG PQVRDWSHYF KIIEDLRAQI FANTVDNARI VLQIDNARLA ADDFRVKYET ELAMRQSVEN DIHGLRKVID DTNITRLQLE TEIEALKEEL LFMKKNHEEE VKGLQAQIAS SGLTVEVDAP KSQDLAKIMA DIRAQYDELA RKNREELDKY WSQQIEESTT VVTTQSAEVG AAETTLTELR RTVQSLEIDL DSMRNLKASL ENSLREVEAR YALQMEQLNG ILLHLESELA QTRAEGQRQA QEYEALLNIK VKLEAEIATY RRLLEDGEDF NLGDALDSSN SMQTIQKTTT RRIVDGKVVS ETNDTKVLRH


    Database link: P05783

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human lung, prostate, thyroid, colon and cervical carcinoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab215904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 2 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

  • Tissue specificity

    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

  • Involvement in disease

    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].

  • Sequence similarities

    Belongs to the intermediate filament family.

  • Post-translational
    modifications

    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.

  • Cellular localization

    Cytoplasm > perinuclear region.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cell proliferation inducing gene 46 protein antibody
      • Cell proliferation inducing protein 46 antibody
      • Cell proliferation-inducing gene 46 protein antibody
      • CK 18 antibody
      • CK-18 antibody
      • CK18 antibody
      • CYK 18 antibody
      • CYK18 antibody
      • Cytokeratin 18 antibody
      • Cytokeratin endo B antibody
      • Cytokeratin-18 antibody
      • K 18 antibody
      • K18 antibody
      • K1C18_HUMAN antibody
      • KA18 antibody
      • Keratin 18 antibody
      • Keratin 18, type I antibody
      • Keratin D antibody
      • keratin, type I cytoskeletal 18 antibody
      • Keratin-18 antibody
      • Krt18 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)

      Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human cervical carcinoma tissue labeling Cytokeratin 18 with ab215904 at 1 μg/ml dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)

      Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human colon carcinoma tissue labeling Cytokeratin 18 with ab215904 at 1 μg/ml dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)

      Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human prostate carcinoma tissue labeling Cytokeratin 18 with ab215904 at 1 μg/ml dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)

      Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human thyroid carcinoma tissue labeling Cytokeratin 18 with ab215904 at 1 μg/ml dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)

      Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human lung carcinoma tissue labeling Cytokeratin 18 with ab215904 at 1 μg/ml dilution.

    References

    ab215904 has not yet been referenced specifically in any publications.

    Publishing research using ab215904? Please let us know so that we can cite the reference in this datasheet.

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