Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)
Key features and details
- Mouse monoclonal [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] to Cytokeratin 18
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190]
See all Cytokeratin 18 primary antibodies -
Description
Mouse monoclonal [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] to Cytokeratin 18 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant full length protein corresponding to Human Cytokeratin 18 aa 1-430.
Sequence:MSFTTRSTFS TNYRSLGSVQ APSYGARPVS SAASVYAGAG GSGSRISVSR STSFRGGMGS GGLATGIAGG LAGMGGIQNE KETMQSLNDR LASYLDRVRS LETENRRLES KIREHLEKKG PQVRDWSHYF KIIEDLRAQI FANTVDNARI VLQIDNARLA ADDFRVKYET ELAMRQSVEN DIHGLRKVID DTNITRLQLE TEIEALKEEL LFMKKNHEEE VKGLQAQIAS SGLTVEVDAP KSQDLAKIMA DIRAQYDELA RKNREELDKY WSQQIEESTT VVTTQSAEVG AAETTLTELR RTVQSLEIDL DSMRNLKASL ENSLREVEAR YALQMEQLNG ILLHLESELA QTRAEGQRQA QEYEALLNIK VKLEAEIATY RRLLEDGEDF NLGDALDSSN SMQTIQKTTT RRIVDGKVVS ETNDTKVLRH
Database link: P05783 -
Positive control
- Human lung, prostate, thyroid, colon and cervical carcinoma tissues.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
ab215904 is purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
KRT18/834, KRT18/835, KRT18/836, KRT18/1190 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab215904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. -
Tissue specificity
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. -
Involvement in disease
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsPhosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. -
Cellular localization
Cytoplasm > perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 3875 Human
- Entrez Gene: 16668 Mouse
- Omim: 148070 Human
- SwissProt: P05783 Human
- SwissProt: P05784 Mouse
- Unigene: 406013 Human
- Unigene: 22479 Mouse
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Alternative names
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
- Cell proliferation-inducing gene 46 protein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human cervical carcinoma tissue labeling Cytokeratin 18 with ab215904 at 1 μg/ml dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human colon carcinoma tissue labeling Cytokeratin 18 with ab215904 at 1 μg/ml dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human prostate carcinoma tissue labeling Cytokeratin 18 with ab215904 at 1 μg/ml dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human thyroid carcinoma tissue labeling Cytokeratin 18 with ab215904 at 1 μg/ml dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human lung carcinoma tissue labeling Cytokeratin 18 with ab215904 at 1 μg/ml dilution.
Datasheets and documents
References (0)
ab215904 has not yet been referenced specifically in any publications.