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    cytokeratin-18-antibody-krt18834-krt18835-krt18836-krt181190-ab215904.pdf

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Tags & Cell Markers Cell Type Markers Tumor Associated
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Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)

Key features and details

  • Mouse monoclonal [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] to Cytokeratin 18
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190]
    See all Cytokeratin 18 primary antibodies
  • Description

    Mouse monoclonal [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] to Cytokeratin 18
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant full length protein corresponding to Human Cytokeratin 18 aa 1-430.
    Sequence:

    MSFTTRSTFS TNYRSLGSVQ APSYGARPVS SAASVYAGAG GSGSRISVSR STSFRGGMGS GGLATGIAGG LAGMGGIQNE KETMQSLNDR LASYLDRVRS LETENRRLES KIREHLEKKG PQVRDWSHYF KIIEDLRAQI FANTVDNARI VLQIDNARLA ADDFRVKYET ELAMRQSVEN DIHGLRKVID DTNITRLQLE TEIEALKEEL LFMKKNHEEE VKGLQAQIAS SGLTVEVDAP KSQDLAKIMA DIRAQYDELA RKNREELDKY WSQQIEESTT VVTTQSAEVG AAETTLTELR RTVQSLEIDL DSMRNLKASL ENSLREVEAR YALQMEQLNG ILLHLESELA QTRAEGQRQA QEYEALLNIK VKLEAEIATY RRLLEDGEDF NLGDALDSSN SMQTIQKTTT RRIVDGKVVS ETNDTKVLRH


    Database link: P05783
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human lung, prostate, thyroid, colon and cervical carcinoma tissues.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.05% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    ab215904 is purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    KRT18/834, KRT18/835, KRT18/836, KRT18/1190
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG - Isotype Control (ab37355)
  • Related Products

    • Recombinant Human Cytokeratin 18 protein (ab211935)

Applications

Our Abpromise guarantee covers the use of ab215904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificity

    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in disease

    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localization

    Cytoplasm > perinuclear region.
  • Target information above from: UniProt accession P05783 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3875 Human
    • Entrez Gene: 16668 Mouse
    • Omim: 148070 Human
    • SwissProt: P05783 Human
    • SwissProt: P05784 Mouse
    • Unigene: 406013 Human
    • Unigene: 22479 Mouse
    • Alternative names

      • Cell proliferation inducing gene 46 protein antibody
      • Cell proliferation inducing protein 46 antibody
      • Cell proliferation-inducing gene 46 protein antibody
      • CK 18 antibody
      • CK-18 antibody
      • CK18 antibody
      • CYK 18 antibody
      • CYK18 antibody
      • Cytokeratin 18 antibody
      • Cytokeratin endo B antibody
      • Cytokeratin-18 antibody
      • K 18 antibody
      • K18 antibody
      • K1C18_HUMAN antibody
      • KA18 antibody
      • Keratin 18 antibody
      • Keratin 18, type I antibody
      • Keratin D antibody
      • keratin, type I cytoskeletal 18 antibody
      • Keratin-18 antibody
      • Krt18 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)

      Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human cervical carcinoma tissue labeling Cytokeratin 18 with ab215904 at 1 μg/ml dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)

      Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human colon carcinoma tissue labeling Cytokeratin 18 with ab215904 at 1 μg/ml dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)

      Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human prostate carcinoma tissue labeling Cytokeratin 18 with ab215904 at 1 μg/ml dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)

      Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human thyroid carcinoma tissue labeling Cytokeratin 18 with ab215904 at 1 μg/ml dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [KRT18/834, KRT18/835, KRT18/836, KRT18/1190] (ab215904)

      Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human lung carcinoma tissue labeling Cytokeratin 18 with ab215904 at 1 μg/ml dilution.

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab215904? Please let us know so that we can cite the reference in this datasheet.

    ab215904 has not yet been referenced specifically in any publications.

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