• Product name
    Anti-Cytokeratin 18 antibody [RGE53]
    See all Cytokeratin 18 primary antibodies
  • Description
    Mouse monoclonal [RGE53] to Cytokeratin 18
  • Host species
  • Specificity
    This antibody reacts exclusively with cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them
  • Tested applications
    Suitable for: ICC/IF, Dot blot, Flow Cyt, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human, Pig, Zebrafish
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Hamster, Dog
  • Immunogen

    Cytoskeletal preparation of cells

  • Positive control
    • This antibody gave a positive signal in HeLa and MCF7 whole cell lysates.



Our Abpromise guarantee covers the use of ab9217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Dot blot 1/100 - 1/1000.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


IHC-Fr 1/100 - 1/200.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).


  • Function
    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificity
    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in disease
    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Post-translational
    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localization
    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all


  • All lanes : Anti-Cytokeratin 18 antibody [RGE53] (ab9217) at 5 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

    Exposure time: 30 seconds
  • Immunofluorescence staining images of 9 day old zebrafish embryos.
    ab9217 reacts with with intestinal epithelium and epidermis. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.0
  • Immunofluorescence staining images of 9 day old zebrafish embryos.
    ab9217 reacts with intestinal epithelium and epidermis. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.
  • ab9217 at a 1/100 dilution staining Cytokeratin 18 in human adipose tissue-derived stromal cells by Immunocytochemistry/ Immunofluorescence.
  • Immunohistochemistry on frozen section of human kidney epithelium
  • Immunohistochemistry on frozen section of human colon
  • Immunohistochemistry on frozen section of swine liver hepatocytes
  • Overlay histogram showing MCF7 cells stained with ab9217 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9217, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.


This product has been referenced in:
  • Montalbano M  et al. Modeling of Hepatocytes Proliferation Isolated from Proximal and Distal Zones from Human Hepatocellular Carcinoma Lesion. PLoS One 11:e0153613 (2016). WB, IHC-P ; Human . Read more (PubMed: 27074018) »
  • Montalbano M  et al. Transformation of primary human hepatocytes in hepatocellular carcinoma. Int J Oncol 48:1205-17 (2016). Read more (PubMed: 26717856) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you for contacting us.

There was error on the datasheet which is being updated. The antibody has been tested for ICC/IF and is fully guaranteed. I am sorry 100% Abreview discount cannot be provided this time. However if you are interested in testing this antibody in Rat or mouse cells the discount code can be provided.

Please let me know if you are interested.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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I apologize for the delay.

Vimentin is a mesenchymal cell marker, ab8978 is tested in Zebrafish. It might be suitable for your purpose.

ab9004, ab9021, ab9217 are anti Cytokeratin 4,7 and 18. Against epithelial cell marker the antibodies is tested in Zebrafish.

Could you more specific to type of blood cell marker you are interested in?

We do not have anti M-Cadherin and Myo-D antibodies that could be used for Zebrafish. This assumption is based on the protein sequence similarity between human and Zebrafish. We unfortunately do not have antibodies against these target that might cross react.

I have attached a Excel file of all Zebrafish specific antibodies with immunogen sequence. This file will help you selecting the antibodies based on cross reactivity due to immunogen sequence homology.

I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.

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