Recombinant
RabMAb

Anti-Cytokeratin 18 antibody [SP69], prediluted (ab101542)

Overview

  • Product name
    Anti-Cytokeratin 18 antibody [SP69], prediluted
    See all Cytokeratin 18 primary antibodies
  • Description
    Rabbit monoclonal [SP69] to Cytokeratin 18, prediluted
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cytokeratin 18 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P05783

  • Positive control
    • Human breast carcinomaand Human prostate carcinoma.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab101542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1. (Prediluted). Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
WB Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.

Target

  • Function
    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificity
    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in disease
    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Post-translational
    modifications
    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localization
    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Images

  • ab101542, prediluted, staining Cytokeratin 18 in Human colon tissue. 

  • Anti-Cytokeratin 18 antibody [SP69] (ab93741) at 1/100 dilution + MCF-7 cell lysate

    Predicted band size: 48 kDa



    This image is derived from the non-prediluted version of this antibody (ab93741).
  • ab101542, prediluted, staining Cytokeratin 18 in Human pancreatic adenocarcinoma tissue. 

  • ab101542, prediluted, staining Cytokeratin 18 in Human breast carcinoma tissue. 

  • ab101542, prediluted, staining Cytokeratin 18 in Human renal cell carcinoma. 

  • ab101542, prediluted, staining Cytokeratin 18 in Human prostate adenocarcinoma tissue. 

  • ab101542, prediluted, staining Cytokeratin 18 in Human stomach tissue. 

  • ab101542, prediluted, staining Cytokeratin 18 in Human breast tissue. 

  • ab101542, prediluted, staining Cytokeratin 18 in Human stomach tissue. 

  • ab101542, prediluted, staining Cytokeratin 18 in Human kidney tissue. 

  • ab101542, prediluted, staining Cytokeratin 18 in Human prostate tissue. 

  • ab101542, prediluted, staining Cytokeratin 18 in Human endometrial adenocarcinoma tissue. 

References

This product has been referenced in:
  • Schütz B  et al. Chemical coding and chemosensory properties of cholinergic brush cells in the mouse gastrointestinal and biliary tract. Front Physiol 6:87 (2015). Read more (PubMed: 25852573) »
  • Xiang J  et al. CXCR4 Protein Epitope Mimetic Antagonist POL5551 Disrupts Metastasis and Enhances Chemotherapy Effect in Triple-Negative Breast Cancer. Mol Cancer Ther 14:2473-85 (2015). Read more (PubMed: 26269605) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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