Key features and details
- Rabbit polyclonal to Cytokeratin 18 (phospho S52)
- Suitable for: WB, ICC/IF, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cytokeratin 18 (phospho S52) antibody
See all Cytokeratin 18 primary antibodies
DescriptionRabbit polyclonal to Cytokeratin 18 (phospho S52)
SpecificityThis antibody detects endogenous levels of Cytokeratin 18 only when phosphorylated at serine 52.
Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
Species reactivityReacts with: Human
A synthesized phosphopeptide derived from human Cytokeratin 18 around the phosphorylation site of serine 52 (STSPFR)
- Hela cells and extracts from HepG2 cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab63393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
|ICC/IF||1/500 - 1/1000.|
FunctionInvolved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Tissue specificityExpressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
Involvement in diseaseDefects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
Sequence similaritiesBelongs to the intermediate filament family.
modificationsPhosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
Cellular localizationCytoplasm > perinuclear region.
- Information by UniProt
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Immunofluorescence analysis of HeLa cells, using ab63393 at a 1:500 dilution.
Left image untreated.Right image treated with phospho peptide.
All lanes : Anti-Cytokeratin 18 (phospho S52) antibody (ab63393) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells
Lane 2 : Extracts from HepG2 cells plus phospho peptide
Predicted band size: 48 kDa
Observed band size: 48 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide is 5-10 ug.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab63393 has not yet been referenced specifically in any publications.