Overview

  • Product name

    Anti-Cytokeratin 19 antibody [A53-B]
    See all Cytokeratin 19 primary antibodies
  • Description

    Mouse monoclonal [A53-B] to Cytokeratin 19
  • Host species

    Mouse
  • Specificity

    This antibody reacts with the rod domain of human Cytokeratin 19, a polypeptide of 40 kDa.
  • Tested applications

    Suitable for: WB, IHC-Fr, ICC/IF, IP, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Cytokeratin 19. Human breast cancer MCF-7 cells (A53-B/A2); Human mammary epithelial organoids (BA17).

  • Positive control

    • MCF-7, HeLa, Hep-G2 cells, breast cancer.
  • General notes

    This antibody is made up of IgG2a, kappa (A53-B/A2) and IgG1, kappa (BA17).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from bioreactor concentrates.
  • Clonality

    Monoclonal
  • Clone number

    A53-B
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab194399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 44 kDa.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
ICC/IF Use a concentration of 1 - 2 µg/ml.
IP Use at 1-2 µg/mg of lysate.
Flow Cyt Use 0.5-1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificity

    Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Developmental stage

    Present in hair follicles at all stages of development.
  • Domain

    This keratin differs from all other IF proteins in lacking the C-terminal tail domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 40 kDa keratin intermediate filament antibody
    • CK 19 antibody
    • CK-19 antibody
    • CK19 antibody
    • Cytokeratin 19 antibody
    • Cytokeratin-19 antibody
    • K19 antibody
    • K1C19_HUMAN antibody
    • K1CS antibody
    • Keratin 19 antibody
    • Keratin type I 40 kD antibody
    • Keratin type I 40kD antibody
    • Keratin type I cytoskeletal 19 antibody
    • Keratin, type I cytoskeletal 19 antibody
    • Keratin, type I, 40 kd antibody
    • Keratin-19 antibody
    • KRT19 antibody
    • MGC15366 antibody
    see all

Images

  • All lanes : Anti-Cytokeratin 19 antibody [A53-B] (ab194399) at 1 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : MCF-7 cell lysate

    Predicted band size: 44 kDa

  • Immunohistochemical analysis of formalin fixed paraffin embedded human colon carcinoma labeling Cytokeratin 19 with ab194399 at 1 μg/mL.

References

ab194399 has not yet been referenced specifically in any publications.

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