Anti-Cytokeratin 19 antibody (ab223259)
Key features and details
- Goat polyclonal to Cytokeratin 19
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-Cytokeratin 19 antibody
See all Cytokeratin 19 primary antibodies -
Description
Goat polyclonal to Cytokeratin 19 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan -
Immunogen
Synthetic peptide corresponding to Human Cytokeratin 19 aa 250-350 (Cysteine residue).
Database link: P08727 -
Positive control
- WB: Human colon cancer lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 44.1 kDa).
1 hour primary incubation is recommended for this product. |
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IHC-P |
Use a concentration of 2.5 µg/ml.
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Notes |
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WB
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 44.1 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 2.5 µg/ml. |
Target
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Function
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
Tissue specificity
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. -
Sequence similarities
Belongs to the intermediate filament family. -
Developmental stage
Present in hair follicles at all stages of development. -
Domain
This keratin differs from all other IF proteins in lacking the C-terminal tail domain. - Information by UniProt
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Database links
- Entrez Gene: 3880 Human
- Entrez Gene: 100173010 Orangutan
- Omim: 148020 Human
- SwissProt: P08727 Human
- SwissProt: Q5R8S9 Orangutan
- Unigene: 654568 Human
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Alternative names
- 40 kDa keratin intermediate filament antibody
- CK 19 antibody
- CK-19 antibody
see all
Images
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Anti-Cytokeratin 19 antibody (ab223259) at 0.03 µg/ml + Human colon cancer lysate at 35 µg
Predicted band size: 44.1 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?Primary incubation: 1 hour
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab223259 has not yet been referenced specifically in any publications.