Application
Immunocytochemistry
Sample
Human Cultured Cells (Breast cancer cell line)
Specification
Breast cancer cell line
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10%
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS with 10% goat serum
Secondary antibody
Name
Non-Abcam antibody was used: Ultravision ONE HRP Polymer (Rabbit anti mouse)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
We routinely fixate cultured cells in methanol for 5 mins at -20 C. This antibody consistently stain K19-positive breast cancer cell lines.
Dr. Rene Villadsen
Verified customer
Submitted Sep 25 2009