Product nameAnti-Cytokeratin 19 antibody [EP1580Y] (Alexa Fluor® 488)
See all Cytokeratin 19 primary antibodies
DescriptionRabbit monoclonal [EP1580Y] to Cytokeratin 19 (Alexa Fluor® 488)
ConjugationAlexa Fluor® 488. Ex: 495nm, Em: 519nm
Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Cytokeratin 19 (C terminal). The exact sequence is proprietary.
- ICC/IF: HeLa. Flow Cyt: HeLa
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Dissociation constant (KD)KD = 3.70 x 10 -10 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-Cytokeratin 19 antibody [EP1580Y] (Alexa Fluor® 647) (ab192980)
- Anti-Cytokeratin 19 antibody [EP1580Y] (HRP) (ab193600)
- Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
- Anti-Cytokeratin 19 antibody [EP1580Y] (Alexa Fluor® 594) (ab203443)
- Anti-Cytokeratin 19 antibody [EP1580Y] (Alexa Fluor® 555) (ab203444)
- Anti-Cytokeratin 19 antibody [EP1580Y] (Alexa Fluor® 568) (ab203445)
- Anti-Cytokeratin 19 antibody [EP1580Y] (Allophycocyanin) (ab224980)
- Anti-Cytokeratin 19 antibody [EP1580Y] (Phycoerythrin) (ab224981)
- Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
Our Abpromise guarantee covers the use of ab192643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionInvolved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificityExpressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
Sequence similaritiesBelongs to the intermediate filament family.
Developmental stagePresent in hair follicles at all stages of development.
DomainThis keratin differs from all other IF proteins in lacking the C-terminal tail domain.
- Information by UniProt
- 40 kDa keratin intermediate filament antibody
- CK 19 antibody
- CK-19 antibody
ab192643 staining Cytokeratin 19 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells
The cells were fixed with 4% formaldehyde (10 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton X-100 for 1hr. The cells were then incubated with ab192643 at a working dilution of 1 in 100 (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1 µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1hr with an AlexaFluor® 594 Goat anti-mouse IgG (H&L - preadsorbed) secondary (ab150120) at 2 μg/ml (shown in pseudo-color red).
Nuclear DNA was labeled in blue with DAPI.
This product gave a positive signal in 100% methanol (5 min) fixed HeLa cells under the same testing conditions.
Image was taken with a Confocal microscope (Leica-microsystems, TCS SP8)
Overlay histogram showing HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab192643 (red line).
The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab192643, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
ab192643 has not yet been referenced specifically in any publications.