Product nameAnti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker
See all Cytokeratin 19 primary antibodies
DescriptionRabbit monoclonal [EP1580Y] to Cytokeratin 19 - Cytoskeleton Marker
Tested applicationsSuitable for: ICC/IF, IHC-Fr, WB, Flow Cyt, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Cytokeratin 19 (C terminal). The exact sequence is proprietary.
Database link: P08727
- WB: HepG2 and NIH/3T3 cell lysates. IHC-P: Human skin, breast carcinoma, kidney carcinoma, endometrial carcinoma and gastric adenocarcinoma tissues. ICC/IF: HepG2 and MCF-7 cells. Flow Cyt: MCF-7 and HeLa cells. IHC-Fr: Mouse salivary gland tissue.
A trial size is available to purchase for this antibody.
See other anti-rabbit secondary antibodies that can be used with this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Dissociation constant (KD)KD = 3.70 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
- Anti-Cytokeratin 19 antibody [EP1580Y] (Alexa Fluor® 488) (ab192643)
- Anti-Cytokeratin 19 antibody [EP1580Y] (Alexa Fluor® 647) (ab192980)
- Anti-Cytokeratin 19 antibody [EP1580Y] (HRP) (ab193600)
- Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
- Anti-Cytokeratin 19 antibody [EP1580Y] (Alexa Fluor® 594) (ab203443)
- Anti-Cytokeratin 19 antibody [EP1580Y] (Alexa Fluor® 555) (ab203444)
- Anti-Cytokeratin 19 antibody [EP1580Y] (Alexa Fluor® 568) (ab203445)
- Anti-Cytokeratin 19 antibody [EP1580Y] (Allophycocyanin) (ab224980)
- Anti-Cytokeratin 19 antibody [EP1580Y] (Phycoerythrin) (ab224981)
Our Abpromise guarantee covers the use of ab52625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/200 - 1/500.
For unpurified, use 1/50.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/50000 - 1/200000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
For unpurified, use 1/10000 - 1/50000.
|Flow Cyt||1/30 - 1/80.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/400 - 1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For unpurified, use at 1/100.
FunctionInvolved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificityExpressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
Sequence similaritiesBelongs to the intermediate filament family.
Developmental stagePresent in hair follicles at all stages of development.
DomainThis keratin differs from all other IF proteins in lacking the C-terminal tail domain.
- Information by UniProt
- 40 kDa keratin intermediate filament antibody
- CK 19 antibody
- CK-19 antibody
Immunohistochemical staining of paraffin-embedded human skin with purified ab52625 at a dilution of 1/400. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunocytochemistry/Immunofluorescence analysis of HepG2 (human liver hepatocellular carcinoma cell line) cells labelling Cytokeratin 19 (green) with purified ab52625 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291, anti-Tubulin (mouse mAb) at 1/1000 followed by ab150120 AlexaFluor®594 goat anti-mouse secondary (1/1000). Nuclei were counterstained with DAPI (blue).
For negative control 1, rabbit primary antibody and anti-mouse secondary antibody (ab150120) were used. For negative control 2, ab7291 (mouse primary antibody) was used followed by anti-rabbit secondary antibody (ab150077).
All lanes : Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625) at 1/45000 dilution (purified)
Lane 1 : HepG2 (liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 44 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
ab52625 staining Cytokeratin 19 in the human cell line MCF-7 (human breast carcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/80. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
ab52625 staining Cytokeratin 19 in Mouse salivary gland by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton and blocked with 2000µg BSA for 1 hour at 21°C. Samples were incubated with primary antibody (PBS + 0.05% Triton + 0.1% BSA) for 24 hours at 4°C. A Alexa Flour® 488 -conjugated goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.
Unpurified ab52625 showing positive staining in Breast carcinoma tissue.
Immunofluorescent staining of MCF-7 cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified ab52625 at a dilution of 1/200. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200 and the cells were counter stained with DAPI.
Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with unpurified ab52625 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52625, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Unpurified ab52625 showing positive staining in Endometrial carcinoma tissue.
Unpurified ab52625 showing negative staining in Glioma tissue.
Unpurified ab52625 showing positive staining in Gastric adenocarcinoma tissue.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Zhou ZQ et al. Adipose extracellular matrix promotes skin wound healing by inducing the differentiation of adipose-derived stem cells into fibroblasts. Int J Mol Med 43:890-900 (2019). Read more (PubMed: 30535488) »
- Shan D et al. Heterozygous Pkhd1C642* mice develop cystic liver disease and proximal tubule ectasia that mimics radiographic signs of medullary sponge kidney. Am J Physiol Renal Physiol 316:F463-F472 (2019). Read more (PubMed: 30600684) »