Recombinant
RabMAb

Recombinant Anti-Cytokeratin 19 antibody [EPNCIR127B] - BSA and Azide free (ab233174)

Overview

  • Product name

    Anti-Cytokeratin 19 antibody [EPNCIR127B] - BSA and Azide free
    See all Cytokeratin 19 primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR127B] to Cytokeratin 19 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to residues at the C-terminus of Mouse Cytokeratin 19.

  • Positive control

    • Mouse kidney, Mouse heart, Mouse spleen, and F9 cell lysates, Mouse colon tissue
  • General notes

    ab233174 is the carrier-free version of ab133496 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    A233174 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed as part of a collaboration between the National Cancer Institutes Center for Cancer Research and the lab of Terry Van Dyke. View antibodies from NCI Center for Cancer Research Collaboration.

    Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab233174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 44 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificity

    Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Developmental stage

    Present in hair follicles at all stages of development.
  • Domain

    This keratin differs from all other IF proteins in lacking the C-terminal tail domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 40 kDa keratin intermediate filament antibody
    • CK 19 antibody
    • CK-19 antibody
    • CK19 antibody
    • Cytokeratin 19 antibody
    • Cytokeratin-19 antibody
    • K19 antibody
    • K1C19_HUMAN antibody
    • K1CS antibody
    • Keratin 19 antibody
    • Keratin type I 40 kD antibody
    • Keratin type I 40kD antibody
    • Keratin type I cytoskeletal 19 antibody
    • Keratin, type I cytoskeletal 19 antibody
    • Keratin, type I, 40 kd antibody
    • Keratin-19 antibody
    • KRT19 antibody
    • MGC15366 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Mouse colon tissue labelling Cytokeratin 19 using unpurified ab133496 antibody at a dilution of 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133496).

  • This IHC data was generated using the same anti-Cytokeratin 19 antibody clone, EPNCIR127B, in a different buffer formulation (cat# ab133496).

    Immunohistochemical analysis of mouse orofacial tissue, staining Cytokeratin 19 with unpurified ab133496.

    Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in Tris-EDTA buffer (pH 9). Samples were incubated with primary antibody (1/500 in diluent) for 24 hours at 4°C. An HRP-conjugated goat anti-rabbit polyclonal IgG (1/2) was used as the secondary antibody.

  • This IHC data was generated using the same anti-Cytokeratin 19 antibody clone, EPNCIR127B, in a different buffer formulation (cat# ab133496).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue sections labeling Cytokeratin 19 with purified ab133496 at 1:500 dilution (1.33 ?g/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

References

ab233174 has not yet been referenced specifically in any publications.

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