For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    cytokeratin-19-antibody-epr1579y-ab76539.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)

  • Datasheet
  • SDS
Reviews (3)Q&A (2)References (7)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)
  • Immunoprecipitation - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)
  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)
  • Western blot - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)
  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)
  • Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1579Y] to Cytokeratin 19
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 APC Carrier Free PE PerCP

You may also be interested in

Primary
Product image
Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)

View more associated products

Overview

  • Product name

    Anti-Cytokeratin 19 antibody [EPR1579Y]
    See all Cytokeratin 19 primary antibodies
  • Description

    Rabbit monoclonal [EPR1579Y] to Cytokeratin 19
  • Host species

    Rabbit
  • Specificity

    In our WB testing this antibody did not recognize mouse Cytokeratin 19, but customers have reported the antibody recognizes the protein in IHC-P with mouse tissue. Thus, we have removed mouse as a "does not react with" species and welcome further feedback from other researchers using this antibody in mouse.
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: T47D, HepG2 or SKBR-3 cell lysate; IHC-P: Human stomach adenocarcinoma tissue. IF/ICC: HCT116 cell line. MCF7 cells (MCF7-KRT19 KO used as negative cell line).
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1579Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EPR1579Y] (ab205445)
    • Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EPR1579Y] (ab205446)
    • PE Anti-Cytokeratin 19 antibody [EPR1579Y] (ab216705)
    • APC Anti-Cytokeratin 19 antibody [EPR1579Y] (ab221255)
    • PerCP Anti-Cytokeratin 19 antibody [EPR1579Y] (ab221256)
    • Anti-Cytokeratin 19 antibody [EPR1579Y] - BSA and Azide free (ab232566)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (1)
1/10000 - 1/50000. Predicted molecular weight: 44 kDa.
IP
1/20.
IHC-P
1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

ICC/IF (1)
1/100 - 1/250.

Signal can be observed in cells fixed with either methanol or paraformaldehyde.

Notes
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/10000 - 1/50000. Predicted molecular weight: 44 kDa.
IP
1/20.
IHC-P
1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

ICC/IF
1/100 - 1/250.

Signal can be observed in cells fixed with either methanol or paraformaldehyde.

Target

  • Function

    Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificity

    Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Developmental stage

    Present in hair follicles at all stages of development.
  • Domain

    This keratin differs from all other IF proteins in lacking the C-terminal tail domain.
  • Target information above from: UniProt accession P08727 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3880 Human
    • Omim: 148020 Human
    • SwissProt: P08727 Human
    • Unigene: 654568 Human
    • Alternative names

      • 40 kDa keratin intermediate filament antibody
      • CK 19 antibody
      • CK-19 antibody
      • CK19 antibody
      • Cytokeratin 19 antibody
      • Cytokeratin-19 antibody
      • K19 antibody
      • K1C19_HUMAN antibody
      • K1CS antibody
      • Keratin 19 antibody
      • Keratin type I 40 kD antibody
      • Keratin type I 40kD antibody
      • Keratin type I cytoskeletal 19 antibody
      • Keratin, type I cytoskeletal 19 antibody
      • Keratin, type I, 40 kd antibody
      • Keratin-19 antibody
      • KRT19 antibody
      • MGC15366 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)

      ab76539 staining Cytokeratin 19 in wild-type MCF7 cells (top panel) and KRT19 knockout MCF7 cells (bottom panel). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab76539 at 1/500 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer tissue sections labeling Cytokeratin 19 with Purified ab76539 at 1:1000 dilution (0.12 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • Immunoprecipitation - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)
      Immunoprecipitation - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)

      ab76539 (purified) at 1:20 dilution (2ug) immunoprecipitating Cytokeratin 19 in SKBR-3 whole cell lysate.
      Lane 1 (input): SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg
      Lane 2 (+): ab76539 & SKBR-3 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76539 in SKBR-3 whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)
      Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)

      Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Cytokeratin 19 with purified ab76539 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

       

    • Western blot - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)
      Western blot - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)
      All lanes : Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539) at 1/10000 dilution (unpurified)

      Lane 1 : T47D cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : SKBR-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 44 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)

      Immunohistochemical staining of human stomach adenocarcinoma tissue with ab76539 (unpurified) at1/100-1/250.

      Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)

      ab76539 (unpurified) stained HCT116 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76539 at 1/200 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43 µM for 1hour at room temperature.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)

      Fluorescent immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab76539 (unpurified). Green- CK19 red-PI.

      Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)
      Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)

      Overlay histogram showing MCF7 cells stained with unpurified ab76539 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76539, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. 

       

       

    • Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)
      Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab76539? Please let us know so that we can cite the reference in this datasheet.

    ab76539 has been referenced in 7 publications.

    • Sun M  et al. Cleavable cellulosic sponge for functional hepatic cell culture and retrieval. Biomaterials 201:16-32 (2019). PubMed: 30784769
    • Maidji E  et al. Replication of CMV in the gut of HIV-infected individuals and epithelial barrier dysfunction. PLoS Pathog 13:e1006202 (2017). PubMed: 28241080
    • Yanagi Y  et al. In vivo and ex vivo methods of growing a liver bud through tissue connection. Sci Rep 7:14085 (2017). IHC ; Rat . PubMed: 29074999
    • Aliagas E  et al. High expression of ecto-nucleotidases CD39 and CD73 in human endometrial tumors. Mediators Inflamm 2014:509027 (2014). IHC . PubMed: 24707115
    • Galvão AM  et al. Immune-endocrine cross-talk in reproductive biology and pathology. Mediators Inflamm 2014:856465 (2014). IHC ; Human . PubMed: 24872600
    • Rodrigues RM  et al. Human skin-derived stem cells as a novel cell source for in vitro hepatotoxicity screening of pharmaceuticals. Stem Cells Dev 23:44-55 (2014). PubMed: 23952781
    • Liu H  et al. Single-cell clones of liver cancer stem cells have the potential of differentiating into different types of tumor cells. Cell Death Dis 4:e857 (2013). IHC-P ; Human . PubMed: 24136221

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-Cytokeratin 19 antibody [EPR1579Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Rabbit Cultured Cells (Primary rabbit liver cells)
    Permeabilization
    Yes - 0.25% Triton X-100
    Specification
    Primary rabbit liver cells
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 27°C
    Fixative
    Paraformaldehyde
    Read More

    DR. Egi Kardia

    Verified customer

    Submitted May 03 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 19 antibody [EPR1579Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rabbit Cell (Liver)
    Permeabilization
    Yes - 0.25% Triton X-100
    Specification
    Liver
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 27°C
    Fixative
    Paraformaldehyde
    Read More

    DR. Egi Kardia

    Verified customer

    Submitted Jun 29 2020

    Western blot abreview for Anti-Cytokeratin 19 antibody [EPR1579Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Cancer Cell lines)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    20 µg
    Specification
    Cancer Cell lines
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 02 2018

    Question

    I'm searching for CK and vimentin antibodies that recognize human proteins but not the corresponding mouse proteins.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 22 2013

    Answer

    Although mouse samples were not recommended based on the western blot results below with these antibodies, there is still the possibility that the negative results in mouse might be due to the limited sample types, especially for the antibodies with high homology (more than 80%) with mouse proteins.
    CK 18
    ab32118: waiting homology and mouse testing
    CK 8
    ab32357: waiting homology, blank WB in mouse brain, heart, lung, muscle, spleen
    CK 7
    ab52870: no homology, no mouse testing performed due to no homology
    CK 19
    ab76539: 80% homology, blank WB in mouse brain, heart, kidney, spleen
    CK 18
    ab133272: 93% homology, blank WB in mouse brain, heart, kidney, spleen, NIH/3T3, Raw264.7
    CK 18
    ab133302: 86% homology, blank WB in mouse brain, heart, kidney, spleen, NIH/3T3, Raw 264.7
    Vimentin
    ab133260: 100% homology, blank WB in mouse brain, heart, kidney, spleen. Non-specific bands in NIH/3T3.
    Epcam
    I could not find any antibodies in our catalog that have low homology with mouse. Your best option is to look for an antibody with an immunogen between human Epcam aa 36-53, the least homologous region of the protein with mouse Epcam. We do provide custom antibody creation in which we can create a rabbit monoclonal antibody with a synthetic peptide in a region of your choosing, please let me know if this is something you are interested in, and I'd be happy to put you in contact with our custom antibody services group.

    Read More

    Kevin Hanson

    Abcam Scientific Support

    Answered on Nov 23 2013

    Question

    Hi, I am looking to buy an antibody to use in IHC and wondered if you can recommend anything specific. I am staining tissue from a mouse which has had human cancer cells injected into it and I am looking to detect metastasis of the human cancer to mouse organs. I am looking for an antibody which will only stain human cancer cells within the mouse tissue; do you have any particular products which best fit this description? My first thought was to use a pan cytokeratin antibody, but I am not sure if any are available that will not also stain mouse cells. Any help would be appreciated.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 14 2011

    Answer

    Thank you very much for contacting us. Following your request, I have searched our catalog for all antibodies that match the mentioned criteria, i.e. that recognise human targets and that do not react with mouse. Please find attached a list of these antibodies. Among these are a number of anti-cytokeratin antibodies (e.g. ab76539, ab76318, and ab108417) which we can guarantee that they do not react with the mouse orthologs, as it has been tested specifically. This is also applicable to other targets, such as MRP1 (ab3369). However, there are a few antibodies on this list which state on the datasheet that they do not react with mouse, but this information is based on sequence similarities only and has not been tested. I would be pleased to let you know though if we can guarantee that the antibody you have chosen does not cross-react with mouse. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Oct 14 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.