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Hi, I am looking to buy an antibody to use in IHC and wondered if you can recommend anything specific. I am staining tissue from a mouse which has had human cancer cells injected into it and I am looking to detect metastasis of the human cancer to mouse organs. I am looking for an antibody which will only stain human cancer cells within the mouse tissue; do you have any particular products which best fit this description? My first thought was to use a pan cytokeratin antibody, but I am not sure if any are available that will not also stain mouse cells. Any help would be appreciated.
Asked on Oct 14 2011
Thank you very much for contacting us. Following your request, I have searched our catalog for all antibodies that match the mentioned criteria, i.e. that recognise human targets and that do not react with mouse. Please find attached a list of these antibodies. Among these are a number of anti-cytokeratin antibodies (e.g. ab76539, ab76318, and ab108417) which we can guarantee that they do not react with the mouse orthologs, as it has been tested specifically. This is also applicable to other targets, such as MRP1 (ab3369). However, there are a few antibodies on this list which state on the datasheet that they do not react with mouse, but this information is based on sequence similarities only and has not been tested. I would be pleased to let you know though if we can guarantee that the antibody you have chosen does not cross-react with mouse. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Oct 14 2011