The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
Prediluted, ready-to-use for 10 min at RT. Staining of formalin-fixed tissues requires digesting tissue sections with pepsin for 10 min at 37°C.
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
Belongs to the intermediate filament family.
Present in hair follicles at all stages of development.
This keratin differs from all other IF proteins in lacking the C-terminal tail domain.
Formalin-fixed Human skin cells stained with ab15464. The antibody is provided ready-to-use. Formalin-fixed tissue sections were digested with Pepsin for 10 mins at 37°C prior to staining with ab15464 for 10 mins at room temperature. .
This product has been referenced in:
Jeong SH et al. Hippo-mediated suppression of IRS2/AKT signaling prevents hepatic steatosis and liver cancer. J Clin Invest128:1010-1025 (2018).
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