Anti-Cytokeratin 19 antibody [RCK108] (ab9221)

Reviews (5)Q&A (2)References (7)

Overview

  • Product name

    Anti-Cytokeratin 19 antibody [RCK108]
    See all Cytokeratin 19 primary antibodies

  • Description

    Mouse monoclonal [RCK108] to Cytokeratin 19

  • Host species

    Mouse

  • Specificity

    This antibody reacts exclusively with cytokeratin 19 which is present in glandular-type epithelia and most carcinomas. It does not react with hepatocytes and hepatocellular carcinoma.

  • Tested applications

    Suitable for: ICC/IF, Dot blot, Flow Cyt, IHC-Fr, ICC, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human, Zebrafish

  • Immunogen

    Human bladder carcinoma cell line T24.

  • General notes


    Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Primary antibody notes

    Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

  • Clonality

    Monoclonal

  • Clone number

    RCK108

  • Myeloma

    Sp2/0-Ag14

  • Isotype

    IgG1

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Dot blot 1/100 - 1/1000.
Flow Cyt 1/100 - 1/200.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-Fr 1/100 - 1/200.
ICC Use at an assay dependent concentration.
IHC-P 1/100 - 1/200.
WB 1/100 - 1/1000.

Target

  • Function

    Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

  • Tissue specificity

    Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.

  • Sequence similarities

    Belongs to the intermediate filament family.

  • Developmental stage

    Present in hair follicles at all stages of development.

  • Domain

    This keratin differs from all other IF proteins in lacking the C-terminal tail domain.
  • Information by UniProt

  • Database links

    • Alternative names

      • 40 kDa keratin intermediate filament antibody
      • CK 19 antibody
      • CK-19 antibody
      • CK19 antibody
      • Cytokeratin 19 antibody
      • Cytokeratin-19 antibody
      • K19 antibody
      • K1C19_HUMAN antibody
      • K1CS antibody
      • Keratin 19 antibody
      • Keratin type I 40 kD antibody
      • Keratin type I 40kD antibody
      • Keratin type I cytoskeletal 19 antibody
      • Keratin, type I cytoskeletal 19 antibody
      • Keratin, type I, 40 kd antibody
      • Keratin-19 antibody
      • KRT19 antibody
      • MGC15366 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
      ab9221 staining Cytokeratin 19 in human skin (sweat glands).
      Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)This image is courtesy of an Abreview submitted by Dr Hannah Whiteman

      Ab9221 staining cells from a human pancreatic cancer cell line by ICC/IF.  The cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C.  Ab9921 was diluted 1/100 and incubated with the sample for 1 hour at 22°C.  An Alexa Fluor® 488 conjugated donkey anti-mouse antibody, diluted 1/1000, was used as the secondary.

      See Abreview

    • Flow Cytometry - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
      Flow Cytometry - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
      Overlay histogram showing MCF-7 cells stained with ab9221 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9221, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

    • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
      Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
      ab9221 staining cytokeratin 19 in three-day-old Zebrafish tissue by Immunohistochemistry (Frozen sections).
    • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
      Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
      Immunohistochemistry on frozen section of human liver bile duct epithelium

    References

    This product has been referenced in:

    See all 7 Publications for this product

    Publishing research using ab9221? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Cytokeratin 19 antibody [RCK108]

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Casein as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 20°C
    Read More

    Dr. Vaughan Feisst

    Verified customer

    Submitted Jun 27 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 19 antibody [RCK108]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Carcinoma)
    Specification
    Carcinoma
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 26 2011

    Western blot abreview for Anti-Cytokeratin 19 antibody [RCK108]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (pancreatic cancer cell line)
    Loading amount
    20 µg
    Specification
    pancreatic cancer cell line
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Hannah Whiteman

    Verified customer

    Submitted Jul 04 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 19 antibody [RCK108]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (pancreatic cancer cell line)
    Specification
    pancreatic cancer cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Read More

    Dr. Hannah Whiteman

    Verified customer

    Submitted Jun 18 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 19 antibody [RCK108]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (MCF-7)
    Specification
    MCF-7
    Fixative
    Acetone
    Permeabilization
    Yes - 0.2% Triton-X-100
    Blocking step
    Fish Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 12 2008

    Question

    KH 20/7/06 Customer phoned. Requires recommended dilution and antigen retrieval methods for ab14521, ab10245, ab9221. Also any suggestions for a good negative control.

    Read More
    Answer

    Thank you for your phone call yesterday regarding antigen retrieval methods for ab9221, ab10245 and ab14521. I have copied a link here for you for our website protocols page on IHC-P and antigen retreival. I hope this will be useful: https://www.abcam.com/assets/pdf/protocols/IHC-paraffin%20protocol%20(IHC-P).pdf Ab9221 against cytokeritin A will require a more gentle pre-treatment with pepsin or trypsin. To our knowledge, the a10245 against cardiac Troponin C has yet to be tested in this application. If you decide to go ahead and purchase this product, we would be very pleased to hear how you get on and if you if you could submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and make all product information available to researchers. If you send us an Abreview, we can offer you from 50 up to 300 Abcam points (Abpoints) based on submission time and type of application. The less characterised the product you review, the more Abpoints you will receive. Furthermore, you get 100 extra Abpoints for submitting an image. These Abpoints can then be claimed back as rewards. You are right, it is hard to find negative controls for these protein targets! There is suggested negative control for ab9221 against cytokeratin 19. Normal Hepatocyte cells or liver tissue should not express cytokeratin 19. Otherwise, you can run a sample with antibody buffer containing no primary antibody as a negative control. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Please, let me know the exact epitope (which aminoacids) against which your monoclonal cytokeratin Abs were raised.

    Read More
    Answer

    We regret to inform you that the epitope of these antibodies have yet not been mapped.

    Read More

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