Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
- Datasheet
- Specific References (6)
- Protocols
Overview
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Product nameAnti-Cytokeratin 19 antibody [RCK108]
See all Cytokeratin 19 primary antibodies -
DescriptionMouse monoclonal [RCK108] to Cytokeratin 19
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Host speciesMouse
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SpecificityThis antibody reacts exclusively with cytokeratin 19 which is present in glandular-type epithelia and most carcinomas. It does not react with hepatocytes and hepatocellular carcinoma.
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Tested applicationsSuitable for: ICC/IF, Dot blot, Flow Cyt, IHC-Fr, ICC, IHC-P, WBmore details
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Species reactivityReacts with: Human, Zebrafish
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Immunogen
Human bladder carcinoma cell line T24.
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General notes
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein A purified
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Primary antibody notesCytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
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ClonalityMonoclonal
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Clone numberRCK108
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MyelomaSp2/0-Ag14
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab9221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| ICC/IF | Use at an assay dependent concentration. | |
| Dot blot | 1/100 - 1/1000. | |
| Flow Cyt | 1/100 - 1/200. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| IHC-Fr | 1/100 - 1/200. | |
| ICC | Use at an assay dependent concentration. | |
| IHC-P | 1/100 - 1/200. | |
| WB | 1/100 - 1/1000. |
Target
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FunctionInvolved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
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Tissue specificityExpressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
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Sequence similaritiesBelongs to the intermediate filament family.
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Developmental stagePresent in hair follicles at all stages of development.
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DomainThis keratin differs from all other IF proteins in lacking the C-terminal tail domain.
- Information by UniProt
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Database links
- Entrez Gene: 3880 Human
- Omim: 148020 Human
- SwissProt: P08727 Human
- Unigene: 654568 Human
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Alternative names
- 40 kDa keratin intermediate filament antibody
- CK 19 antibody
- CK-19 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)](https://www.abcam.com/ps/products/9/ab9221/Images/ab9221_3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)This image is courtesy of an Abreview submitted by Dr Hannah WhitemanAb9221 staining cells from a human pancreatic cancer cell line by ICC/IF. The cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C. Ab9921 was diluted 1/100 and incubated with the sample for 1 hour at 22°C. An Alexa Fluor® 488 conjugated donkey anti-mouse antibody, diluted 1/1000, was used as the secondary.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)](https://www.abcam.com/ps/products/9/ab9221/Images/Cytokeratin-19-Primary-antibodies-ab9221-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)ab9221 staining Cytokeratin 19 in human skin (sweat glands).
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
![Flow Cytometry - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)](https://www.abcam.com/ps/products/9/ab9221/Images/Cytokeratin-19-Primary-antibodies-ab9221-2.jpg)
Flow Cytometry - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)Overlay histogram showing MCF-7 cells stained with ab9221 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9221, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions. -
![Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)](https://www.abcam.com/ps/products/9/ab9221/Images/Cytokeratin-19-Primary-antibodies-ab9221-6.jpg)
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)ab9221 staining cytokeratin 19 in three-day-old Zebrafish tissue by Immunohistochemistry (Frozen sections). -
![Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)](https://www.abcam.com/ps/products/9/ab9221/Images/Cytokeratin-19-Primary-antibodies-ab9221-7.jpg)
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)Immunohistochemistry on frozen section of human liver bile duct epithelium
Protocols
References
This product has been referenced in:
- Wang J et al. The construction of intrahepatic cholangiocarcinoma model in zebrafish. Sci Rep 7:13419 (2017). Read more (PubMed: 29042681) »
- Huang L et al. Ductal pancreatic cancer modeling and drug screening using human pluripotent stem cell- and patient-derived tumor organoids. Nat Med 21:1364-71 (2015). Read more (PubMed: 26501191) »
Customer reviews and Q&As
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