Anti-Cytokeratin 19 antibody [SB39g] (FITC) (ab87014)
Key features and details
- Mouse monoclonal [SB39g] to Cytokeratin 19 (FITC)
- Suitable for: Flow Cyt, ICC
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Overview
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Product name
Anti-Cytokeratin 19 antibody [SB39g] (FITC)
See all Cytokeratin 19 primary antibodies -
Description
Mouse monoclonal [SB39g] to Cytokeratin 19 (FITC) -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cyt, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment (Human)
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Positive control
- Flow Cyt: HT29 cells ELISA and IHC: HT29 and HCC38 cells WB: HT29 and HCC38 cell lysates
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General notes
Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.
This product was changed from ascites to tissue culture supernatant on 17th September 2019. Lot numbers higher than GR3224453 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Primary antibody notes
Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. -
Clonality
Monoclonal -
Clone number
SB39g -
Isotype
IgG2a -
Research areas
Associated products
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab87014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| ICC | Use at an assay dependent concentration. |
Target
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Function
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
Tissue specificity
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. -
Sequence similarities
Belongs to the intermediate filament family. -
Developmental stage
Present in hair follicles at all stages of development. -
Domain
This keratin differs from all other IF proteins in lacking the C-terminal tail domain. - Information by UniProt
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Database links
- Entrez Gene: 3880 Human
- Omim: 148020 Human
- SwissProt: P08727 Human
- Unigene: 654568 Human
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Alternative names
- 40 kDa keratin intermediate filament antibody
- CK 19 antibody
- CK-19 antibody
see all
Images
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![Flow Cytometry - Anti-Cytokeratin 19 antibody [SB39g] (FITC) (ab87014)](https://www.abcam.com/ps/products/87/ab87014/Images/ab87014-102062-anti-cytokeratin-19-antibody-sb39g-fitc-flow-cytometry.jpg)
Flow Cytometry - Anti-Cytokeratin 19 antibody [SB39g] (FITC) (ab87014)Overlay histogram showing MCF-7 cells stained with ab87014 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87014, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a (FITC) (1µg/1x106 cells) for 30 min at 22°C. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
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![Immunocytochemistry - Anti-Cytokeratin 19 antibody [SB39g] (FITC) (ab87014)](https://www.abcam.com/ps/products/87/ab87014/Images/ab87014-289103-anti-cytokeratin-19-antibody-sb39g-fitc-immunocytochemistry.jpg)
Immunocytochemistry - Anti-Cytokeratin 19 antibody [SB39g] (FITC) (ab87014)Ab87014 staining Cytokeratin 19 in MIA PaCa-2 cells ( Human pancreatic carcinoma cell line) .
This image was generated using the ascites version of the product.
Protocols
References (4)
ab87014 has been referenced in 4 publications.
- Yue C et al. Dynamic change of PD-L1 expression on circulating tumor cells in advanced solid tumor patients undergoing PD-1 blockade therapy. Oncoimmunology 7:e1438111 (2018). PubMed: 29900038
- Zhou M et al. Precisely Enumerating Circulating Tumor Cells Utilizing a Multi-Functional Microfluidic Chip and Unique Image Interpretation Algorithm. Theranostics 7:4710-4721 (2017). PubMed: 29187898
- Santoro A et al. Relationship between CK19 expression, deregulation of normal keratinocyte differentiation pattern and high risk-human papilloma virus infection in oral and oropharyngeal squamous cell carcinoma. Infect Agent Cancer 10:46 (2015). Flow Cyt . PubMed: 26672675
- Cardinale V et al. Profiles of cancer stem cell subpopulations in cholangiocarcinomas. Am J Pathol 185:1724-39 (2015). PubMed: 25892683
