Anti-Cytokeratin 19 antibody [SB39g] (FITC) (ab87014)

Overlay histogram showing MCF-7 cells stained with ab87014 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87014, 0.5µg/1x10⁶ cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a (FITC) (1µg/1x10⁶ cells) for 30 min at 22°C. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.