Anti-Cytokeratin 19 antibody [SB39g] (FITC) (ab87014)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-Cytokeratin 19 antibody [SB39g] (FITC)
See all Cytokeratin 19 primary antibodies -
Description
Mouse monoclonal [SB39g] to Cytokeratin 19 (FITC) -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: WB, ELISA, Flow Cyt, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment (Human)
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Positive control
- FC: HT29 cells ELISA and IHC: HT29 and HCC38 cells WB: HT29 and HCC38 cell lysates
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General notes
Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.
This product was changed from ascites to tissue culture supernatant on 17th September 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Purity
Tissue culture supernatant -
Primary antibody notes
Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. -
Clonality
Monoclonal -
Clone number
SB39g -
Isotype
IgG2a -
Research areas
Associated products
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab87014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use at an assay dependent concentration. Predicted molecular weight: 44 kDa. | |
ELISA | Use at an assay dependent concentration. | |
Flow Cyt | Use at an assay dependent concentration. ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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ICC | Use at an assay dependent concentration. |
Target
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Function
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
Tissue specificity
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. -
Sequence similarities
Belongs to the intermediate filament family. -
Developmental stage
Present in hair follicles at all stages of development. -
Domain
This keratin differs from all other IF proteins in lacking the C-terminal tail domain. - Information by UniProt
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Database links
- Entrez Gene: 3880 Human
- Omim: 148020 Human
- SwissProt: P08727 Human
- Unigene: 654568 Human
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Alternative names
- 40 kDa keratin intermediate filament antibody
- CK 19 antibody
- CK-19 antibody
see all
Images
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Overlay histogram showing MCF-7 cells stained with ab87014 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87014, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a (FITC) (1µg/1x106 cells) for 30 min at 22°C. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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Ab87014 staining Cytokeratin 19 in MIA PaCa-2 cells ( Human pancreatic carcinoma cell line) .
References
This product has been referenced in:
- Yue C et al. Dynamic change of PD-L1 expression on circulating tumor cells in advanced solid tumor patients undergoing PD-1 blockade therapy. Oncoimmunology 7:e1438111 (2018). Read more (PubMed: 29900038) »
- Zhou M et al. Precisely Enumerating Circulating Tumor Cells Utilizing a Multi-Functional Microfluidic Chip and Unique Image Interpretation Algorithm. Theranostics 7:4710-4721 (2017). Read more (PubMed: 29187898) »