Product nameCytokeratin 19 overexpression 293T lysate (whole cell)
See all Cytokeratin 19 lysates
DescriptionCytokeratin 19 overexpression lysate
ab94060 is a 293T cell transfected lysate in which Human Cytokeratin 19 has been transiently over-expressed using a pCMV-Cytokeratin 19 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
Concentration information loading...
BackgroundDevelopmental stage: Present in hair follicles at all stages of development. Domain: This keratin differs from all other IF proteins in lacking the C-terminal tail domain. Function: Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Similarity: Belongs to the intermediate filament family. Tissue specificity: Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
Our Abpromise guarantee covers the use of ab94060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94060 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-Cytokeratin 19 antibody (ab90440) at 1/500 dilution
Lane 1 :
Cytokeratin 19 overexpression 293T lysate (whole cell) (ab94060)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94060 has not yet been referenced specifically in any publications.