Key features and details
- Rabbit polyclonal to Cytokeratin 2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cytokeratin 2 antibody
See all Cytokeratin 2 primary antibodies
DescriptionRabbit polyclonal to Cytokeratin 2
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- WB: PC-3 whole cell lysate. IHC-P: Human endometrial cancer tissue. ICC/IF: SH-SY5Y cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500.|
|WB||1/500 - 1/5000. Predicted molecular weight: 66 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionProbably contributes to terminal cornification.
Sequence similaritiesBelongs to the intermediate filament family.
Developmental stageSynthesized during maturation of epidermal keratinocytes.
- Information by UniProt
- CK 2P antibody
- CK-2P antibody
- CK2P antibody
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling Cytokeratin 2 (green) with ab236751 at 1/133 dilution in ICC/IF. Counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L).
Anti-Cytokeratin 2 antibody (ab236751) at 1/500 dilution + PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 66 kDa
Paraffin-embedded human endometrial cancer tissue stained for Cytokeratin 2 using ab236751 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236751 has not yet been referenced specifically in any publications.