Key features and details
- Rabbit polyclonal to Cytokeratin 20 - Cytoskeleton Marker
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Cytokeratin 20 antibody - Cytoskeleton Marker
See all Cytokeratin 20 primary antibodies
DescriptionRabbit polyclonal to Cytokeratin 20 - Cytoskeleton Marker
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment, corresponding to a region within amino acids 75-287 of Human Cytokeratin 20 (NP_061883).
- HeLa cell, HT29 xenograft
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 48 kDa.|
|IHC-P||1/100 - 1/500.|
|ICC/IF||1/100 - 1/200.|
FunctionPlays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
Tissue specificityExpressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
Sequence similaritiesBelongs to the intermediate filament family.
Developmental stageFirst detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.
modificationsHyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
- Information by UniProt
- CD20 antibody
- CK 20 antibody
- CK-20 antibody
Ab97511 staining Cytokeratin 20 in Mouse urinary bladder by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin embedded sections). Sample was incubated with primary antibody at a 1:500 dilution.
Anti-Cytokeratin 20 antibody - Cytoskeleton Marker (ab97511) at 1/3000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 48 kDa
10% SDS PAGE
Ab97511 staining Cytokeratin 20 in HT29 xenograft by Immunohistochemistry (IHC-P - paraformaldehyde - fixed, paraffin embedded sections). Samples were incubated with primary antibody at 1:100 dilution.
Immunofluorescence analysis of Cytokeratin 20 in paraformaldehyde fixed HeLa, using ab97511 at a 1/200 dilution.
Lower image (2): merged with DNA probe.
ab97511 has been referenced in 2 publications.
- Fasano M et al. Small bowel metastasis from pancreatic cancer in a long-term survival patient with synchronous advanced malignant pleural mesothelioma: A case report and literature review. Oncol Lett 12:4505-4509 (2016). IHC-P ; Human . PubMed: 28105159
- Liu H et al. Single-cell clones of liver cancer stem cells have the potential of differentiating into different types of tumor cells. Cell Death Dis 4:e857 (2013). IHC-P ; Human . PubMed: 24136221