Product nameAnti-Cytokeratin 20 antibody [EPR1621(2)]
See all Cytokeratin 20 primary antibodies
DescriptionRabbit monoclonal [EPR1621(2)] to Cytokeratin 20
Tested applicationsSuitable for: Flow Cyt, ICC/IF, WB, IPmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human Cytokeratin 20 (P35900).
- WB: SW480, Mouse small intestine and Rat small intestine lysates ICC/IF: SW480 cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|ICC/IF||1/50 - 1/100.|
|WB||1/10000 - 1/50000. Detects a band of approximately 46 kDa (predicted molecular weight: 48 kDa).|
|IP||1/10 - 1/100.|
FunctionPlays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
Tissue specificityExpressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
Sequence similaritiesBelongs to the intermediate filament family.
Developmental stageFirst detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.
modificationsHyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
- Information by UniProt
- CD20 antibody
- CK 20 antibody
- CK-20 antibody
Flow Cytometry analysis of SW480 (human colorectal adenocarcinoma ) cells labeling Cytokeratin 20 with unpurified ab109111 at 1:20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
All lanes : Anti-Cytokeratin 20 antibody [EPR1621(2)] (ab109111) at 1/10000 dilution
Lane 1 : SW480 lysate
Lane 2 : Mouse small intestine lysate
Lane 3 : Rat small intestine lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Immunofluorescent staining of Cytokeratin 20 in SW480 cells, using ab109111 at a 1/50 dilution.
ab109111 has not yet been referenced specifically in any publications.