Key features and details
- Mouse monoclonal [KRT20/1992] to Cytokeratin 20
- Suitable for: Protein Array, WB, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-Cytokeratin 20 antibody [KRT20/1992]
See all Cytokeratin 20 primary antibodies
DescriptionMouse monoclonal [KRT20/1992] to Cytokeratin 20
Tested applicationsSuitable for: Protein Array, WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Cytokeratin 20 aa 196-323. The exact sequence is proprietary.
Database link: P35900
- WB: HT-29 cell lysate. IHC-P: Human colon and colon carcinoma tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from Bioreactor Concentrate by Protein A/G.
Light chain typekappa
Our Abpromise guarantee covers the use of ab238034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Protein Array||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 48 kDa.|
|IHC-P||Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Incubate with primary antibody for 30 minutes at room temperature.
FunctionPlays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
Tissue specificityExpressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
Sequence similaritiesBelongs to the intermediate filament family.
Developmental stageFirst detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.
modificationsHyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
- Information by UniProt
- CD20 antibody
- CK 20 antibody
- CK-20 antibody
ab238034 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Anti-Cytokeratin 20 antibody [KRT20/1992] (ab238034) at 2 µg/ml + HT-29 (human colorectal adenocarcinoma cell line) cell lysate
Predicted band size: 48 kDa
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Cytokeratin 20 using ab238034 at 2 μg/ml in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human colon tissue stained for Cytokeratin 20 using ab238034 at 2 μg/ml in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238034 has been referenced in 1 publication.
- Zhou JY et al. Hydrolyzed wheat gluten alleviates deoxynivalenol-induced intestinal injury by promoting intestinal stem cell proliferation and differentiation via upregulation of Wnt/ß-catenin signaling in mice. Food Chem Toxicol 131:110579 (2019). PubMed: 31202940