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  1. Link

    cytokeratin-20-antibody-krt201993-ab268107.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins
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Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)
  • Western blot - Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)
  • Protein Array - Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)

Key features and details

  • Mouse monoclonal [KRT20/1993] to Cytokeratin 20
  • Suitable for: WB, IHC-P, Protein Array
  • Reacts with: Human
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-Cytokeratin 20 antibody [KRT20/1993]
    See all Cytokeratin 20 primary antibodies
  • Description

    Mouse monoclonal [KRT20/1993] to Cytokeratin 20
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Protein Arraymore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Cytokeratin 20 aa 196-323. The exact sequence is proprietary.
    Database link: P35900

  • Positive control

    • WB: HT29 cell lysate. IHC-P: Human colon and colon carcinoma tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from bioreactor concentrate.
  • Clonality

    Monoclonal
  • Clone number

    KRT20/1993
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins
    • Cancer
    • Tumor biomarkers
    • Other

Associated products

  • Alternative Versions

    • Anti-Cytokeratin 20 antibody [KRT20/1993] - BSA and Azide free (ab268228)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab268107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 48 kDa.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protein Array
Use at an assay dependent concentration.
Notes
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 48 kDa.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protein Array
Use at an assay dependent concentration.

Target

  • Function

    Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
  • Tissue specificity

    Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Developmental stage

    First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.
  • Post-translational
    modifications

    Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
    Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P35900 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 54474 Human
    • Omim: 608218 Human
    • SwissProt: P35900 Human
    • Unigene: 84905 Human
    • Alternative names

      • CD20 antibody
      • CK 20 antibody
      • CK-20 antibody
      • CK20 antibody
      • Cytokeratin-20 antibody
      • Cytokeratin20 antibody
      • K1C20_HUMAN antibody
      • K20 antibody
      • KA20 antibody
      • Keratin 20 antibody
      • keratin 20, type I antibody
      • keratin 21, rat, homolog of antibody
      • Keratin antibody
      • Keratin type I cytoskeletal 20 antibody
      • Keratin-20 antibody
      • Keratin20 antibody
      • KRT 20 antibody
      • KRT 21 antibody
      • KRT20 antibody
      • KRT21 antibody
      • MGC35423 antibody
      • OTTHUMP00000164518 antibody
      • Protein IT antibody
      • type I cytoskeletal 20 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)

      Formalin-fixed, paraffin-embedded human normal colon tissue stained for Cytokeratin 20 using ab268107 at 2 μg/ml in immunohistochemical analysis. 

    • Western blot - Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)
      Western blot - Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)
      Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107) at 2 µg/ml + HT29 (Human colorectal adenocarcinoma cell line) cell lysate

      Predicted band size: 48 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)

      Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Cytokeratin 20 using ab268107 at 2 μg/ml in immunohistochemical analysis. 

    • Protein Array - Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)
      Protein Array - Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)

      Protein Array containing more than 19,000 full-length human proteins using ab268107.

      Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab268107? Please let us know so that we can cite the reference in this datasheet.

    ab268107 has not yet been referenced specifically in any publications.

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