Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)
- Datasheet
- References (3)
- Protocols
Overview
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Product nameAnti-Cytokeratin 20 antibody [Ks20.8]
See all Cytokeratin 20 primary antibodies -
DescriptionMouse monoclonal [Ks20.8] to Cytokeratin 20
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Host speciesMouse
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SpecificityThis antibody is highly specific to cytokeratin 20 and shows no cross-reaction with other intermediate filament proteins. It is essentially non-reactive in squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary and small cell carcinomas.
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Tested applicationsSuitable for: Flow Cyt, WB, IHC-Fr, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Cytoskeletal preparation from micro-dissected villi of human duodenal mucosa.
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Positive control
- Colon carcinoma. IF/ICC: Jeg3 cell line.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, Carrier protein, Van Gogh Yellow Diluent, pH 7.3 -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberKs20.8
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Myelomaunknown
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IsotypeIgG2a
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Light chain typeunknown
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/50. (Also see PMID: 20332776)
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| WB | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. We suggest an incubation period of 30-60 minutes at room temperature. | |
| ICC/IF | 1/200. |
Target
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FunctionPlays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
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Tissue specificityExpressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
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Sequence similaritiesBelongs to the intermediate filament family.
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Developmental stageFirst detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.
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Post-translational
modificationsHyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. -
Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 54474 Human
- Omim: 608218 Human
- SwissProt: P35900 Human
- Unigene: 84905 Human
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Alternative names
- CD20 antibody
- CK 20 antibody
- CK-20 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)](https://www.abcam.com/ps/products/0/ab854/Images/ab854-197817-ab854jeg3p1in200edit.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)ICC/IF image of ab854 stained Jeg3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab854, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)](https://www.abcam.com/ps/products/0/ab854/Images/Cytokeratin-20-Primary-antibodies-ab854-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)ab854 staining cytokeratin in human colon cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).
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![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)](https://www.abcam.com/ps/products/0/ab854/Images/ab854-252443-ab854IHCfr.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)Immunofluorescent analysis of human colon epithelium, staining Cytokeratin 20 with ab854.
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![Flow Cytometry - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)](https://www.abcam.com/ps/products/0/ab854/Images/Cytokeratin-20-Primary-antibodies-ab854-3.jpg)
Flow Cytometry - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)Overlay histogram showing LoVo cells stained with ab854 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab854, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
References
This product has been referenced in:
- Wang Q et al. Ketogenesis contributes to intestinal cell differentiation. Cell Death Differ 24:458-468 (2017). Read more (PubMed: 27935584) »
- Fridriksdottir AJ et al. Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture. Nat Commun 6:8786 (2015). Read more (PubMed: 26564780) »
