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    cytokeratin-20-antibody-ks208-ab854.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins
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Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)

  • Datasheet
  • SDS
Reviews (4)Q&A (2)References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)
  • Flow Cytometry - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)

Key features and details

  • Mouse monoclonal [Ks20.8] to Cytokeratin 20
  • Suitable for: Flow Cyt, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-Cytokeratin 20 antibody [Ks20.8]
    See all Cytokeratin 20 primary antibodies
  • Description

    Mouse monoclonal [Ks20.8] to Cytokeratin 20
  • Host species

    Mouse
  • Specificity

    This antibody is highly specific to cytokeratin 20 and shows no cross-reaction with other intermediate filament proteins. It is essentially non-reactive in squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary and small cell carcinomas.
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Cytokeratin 20. Electrophoretically purified Keratin K20 from Human intestinal mucosa.

  • Positive control

    • Colon carcinoma. IF/ICC: Jeg3 cell line.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.1% Sodium azide
    Constituents: PBS, Carrier protein

    Van Gogh Yellow Diluent
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    Ks20.8
  • Myeloma

    unknown
  • Isotype

    IgG2a
  • Light chain type

    unknown
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins
    • Cancer
    • Tumor biomarkers
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/50.

(Also see PMID: 20332776)

 

 

 

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

IHC-P (1)
Use at an assay dependent concentration. This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. We suggest an incubation period of 30-60 minutes at room temperature.
ICC/IF (1)
1/200.
Notes
Flow Cyt
1/50.

(Also see PMID: 20332776)

 

 

 

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

IHC-P
Use at an assay dependent concentration. This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. We suggest an incubation period of 30-60 minutes at room temperature.
ICC/IF
1/200.

Target

  • Function

    Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
  • Tissue specificity

    Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Developmental stage

    First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.
  • Post-translational
    modifications

    Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
    Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P35900 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 54474 Human
    • Omim: 608218 Human
    • SwissProt: P35900 Human
    • Unigene: 84905 Human
    • Alternative names

      • CD20 antibody
      • CK 20 antibody
      • CK-20 antibody
      • CK20 antibody
      • Cytokeratin-20 antibody
      • Cytokeratin20 antibody
      • K1C20_HUMAN antibody
      • K20 antibody
      • KA20 antibody
      • Keratin 20 antibody
      • keratin 20, type I antibody
      • keratin 21, rat, homolog of antibody
      • Keratin antibody
      • Keratin type I cytoskeletal 20 antibody
      • Keratin-20 antibody
      • Keratin20 antibody
      • KRT 20 antibody
      • KRT 21 antibody
      • KRT20 antibody
      • KRT21 antibody
      • MGC35423 antibody
      • OTTHUMP00000164518 antibody
      • Protein IT antibody
      • type I cytoskeletal 20 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)

      ab854 staining cytokeratin in human colon cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)

      ICC/IF image of ab854 stained Jeg3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab854, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)

      Immunofluorescent analysis of human colon epithelium, staining Cytokeratin 20 with ab854.

    • Flow Cytometry - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)
      Flow Cytometry - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)
      Overlay histogram showing LoVo cells stained with ab854 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab854, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab854? Please let us know so that we can cite the reference in this datasheet.

    ab854 has been referenced in 3 publications.

    • Wang Q  et al. Ketogenesis contributes to intestinal cell differentiation. Cell Death Differ 24:458-468 (2017). PubMed: 27935584
    • Fridriksdottir AJ  et al. Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture. Nat Commun 6:8786 (2015). PubMed: 26564780
    • Fang DD  et al. Expansion of CD133(+) colon cancer cultures retaining stem cell properties to enable cancer stem cell target discovery. Br J Cancer : (2010). Flow Cyt ; Human . PubMed: 20332776

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-6 of 6 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 20 antibody [Ks20.8]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin (Merkel cell carcinoma))
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: Proteinase K
    Permeabilization
    Yes - 0,2% Triton/PBS
    Specification
    skin (Merkel cell carcinoma)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 06 2018

    Western blot abreview for Anti-Cytokeratin 20 antibody [Ks20.8]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (skin biopsie)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (5-16%)
    Loading amount
    30 µg
    Specification
    skin biopsie
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 06 2018

    Western blot abreview for Anti-Cytokeratin 20 antibody [Ks20.8]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Colon Carcinoma)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    30 µg
    Specification
    Colon Carcinoma
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 22 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 20 antibody [Ks20.8]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Organoid)
    Permeabilization
    Yes - 0.3% Triton
    Specification
    Organoid
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 10 2016

    Question

    Dear colleagues

    One our customer bought Abcam cat# AB854 lot# GR94926-1, and she did note that this antibody was resospended in a almost red buffer: is it normal? Can you guarantee that this strange colour will not affect the performances?

    Please note that the same customer bough, several months ago, the same antibody (but with different lot number) and the buffer within was transparent.

    Thanks a lot

    Read More

    Abcam community

    Verified customer

    Asked on Oct 01 2012

    Answer

    Thank you for your enquiry.

    I can confirm that ab854 Anti-Cytokeratin 20 antibody [Ks20.8]is provided as tissue culture supernatant, which we would expect to have a pink-red coloration. Therefore, I would not expect this to affect the antibody.

    The last vial purchased may have been purified and provided in clear buffer.

    I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Oct 01 2012

    Question

    I am looking to purchase a monoclonal antibody to cytokeratin 20 for use in ELISA. Is this product, ab854, suitable for use in that application?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 27 2004

    Answer

    This antibody has been tested for use in IHC, but to our knowledge has not yet been used in ELISA.

    Read More

    Abcam Scientific Support

    Answered on Oct 27 2004

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