Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)](https://www.abcam.com/ps/products/0/ab854/Images/ab854-3795-anti-cytokeratin-20-antibody-ks208-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)")
Key features and details
- Mouse monoclonal [Ks20.8] to Cytokeratin 20
- Suitable for: Flow Cyt, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
Overview
-
Product name
Anti-Cytokeratin 20 antibody [Ks20.8]
See all Cytokeratin 20 primary antibodies -
Description
Mouse monoclonal [Ks20.8] to Cytokeratin 20 -
Host species
Mouse -
Specificity
This antibody is highly specific to cytokeratin 20 and shows no cross-reaction with other intermediate filament proteins. It is essentially non-reactive in squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary and small cell carcinomas. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P Human
-
Immunogen
Cytoskeletal preparation from micro-dissected villi of human duodenal mucosa.
-
Positive control
- Colon carcinoma. IF/ICC: Jeg3 cell line.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.1% Sodium azide
Constituents: PBS, Carrier protein
Van Gogh Yellow Diluent -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
Ks20.8 -
Myeloma
unknown -
Isotype
IgG2a -
Light chain type
unknown -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/50.
(Also see PMID: 20332776)
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
|
| IHC-P | (1) |
Use at an assay dependent concentration. This antibody may be diluted to a titer of 1:50-1:100 in an ABC method.
We suggest an incubation period of 30-60 minutes at room temperature.
|
| ICC/IF | (1) |
1/200.
|
| Notes |
|---|
|
Flow Cyt
1/50. (Also see PMID: 20332776)
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
|
IHC-P
Use at an assay dependent concentration. This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. We suggest an incubation period of 30-60 minutes at room temperature. |
|
ICC/IF
1/200. |
Target
-
Function
Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine. -
Tissue specificity
Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa. -
Sequence similarities
Belongs to the intermediate filament family. -
Developmental stage
First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative. -
Post-translational
modificationsHyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 54474 Human
- Omim: 608218 Human
- SwissProt: P35900 Human
- Unigene: 84905 Human
-
Alternative names
- CD20 antibody
- CK 20 antibody
- CK-20 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)
ab854 staining cytokeratin in human colon cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).
-
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)](https://www.abcam.com/ps/products/0/ab854/Images/ab854-197817-anti-cytokeratin-20-antibody-ks208-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)ICC/IF image of ab854 stained Jeg3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab854, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
-
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)](https://www.abcam.com/ps/products/0/ab854/Images/ab854-252443-anti-cytokeratin-20-antibody-ks208-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)Immunofluorescent analysis of human colon epithelium, staining Cytokeratin 20 with ab854.
-
![Flow Cytometry - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)](https://www.abcam.com/ps/products/0/ab854/Images/ab854-3794-anti-cytokeratin-20-antibody-ks208-flow-cytometry.jpg)
Flow Cytometry - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)Overlay histogram showing LoVo cells stained with ab854 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab854, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab854 has been referenced in 3 publications.
- Wang Q et al. Ketogenesis contributes to intestinal cell differentiation. Cell Death Differ 24:458-468 (2017). PubMed: 27935584
- Fridriksdottir AJ et al. Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture. Nat Commun 6:8786 (2015). PubMed: 26564780
- Fang DD et al. Expansion of CD133(+) colon cancer cultures retaining stem cell properties to enable cancer stem cell target discovery. Br J Cancer : (2010). Flow Cyt ; Human . PubMed: 20332776