Recombinant
RabMAb

Anti-Cytokeratin 20 antibody [SP33], prediluted (ab64091)

Overview

  • Product name
    Anti-Cytokeratin 20 antibody [SP33], prediluted
    See all Cytokeratin 20 primary antibodies
  • Description
    Rabbit monoclonal [SP33] to Cytokeratin 20, prediluted
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cytokeratin 20 (C terminal). The exact sequence is proprietary.
    Database link: P35900

  • Positive control
    • Human colon carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab64091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Ready-to-use for 30 min at RT.

Target

  • Function
    Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
  • Tissue specificity
    Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Developmental stage
    First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.
  • Post-translational
    modifications
    Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
    Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD20 antibody
    • CK 20 antibody
    • CK-20 antibody
    • CK20 antibody
    • Cytokeratin-20 antibody
    • Cytokeratin20 antibody
    • K1C20_HUMAN antibody
    • K20 antibody
    • KA20 antibody
    • Keratin 20 antibody
    • keratin 20, type I antibody
    • keratin 21, rat, homolog of antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 20 antibody
    • Keratin-20 antibody
    • Keratin20 antibody
    • KRT 20 antibody
    • KRT 21 antibody
    • KRT20 antibody
    • KRT21 antibody
    • MGC35423 antibody
    • OTTHUMP00000164518 antibody
    • Protein IT antibody
    • type I cytoskeletal 20 antibody
    see all

Images

References

ab64091 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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