Overview

  • Product name

    Anti-Cytokeratin 2e antibody
    See all Cytokeratin 2e primary antibodies
  • Description

    Rabbit polyclonal to Cytokeratin 2e
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Fusion protein corresponding to Human Cytokeratin 2e (internal sequence). (BC096294).
    Database link: P35908

  • Positive control

    • Jurkat cell lysate; Human ovarian cancer tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab197365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/100.
WB 1/200 - 1/1000. Predicted molecular weight: 65 kDa.

Target

  • Relevance

    Keratins are the major gene product of keratinocytes and form the intermediate filament cytoskeletal network in these cells. In cells of the upper spinous layer, KRT2E and KRT9 are expressed. Although the expression of KRT9 is limited to palmoplantar epidermis, KRT2E is expressed not only in this tissue but also in other regions, notably the epidermis covering the knee, thigh, and groin. It is not known whether these keratins simply replace their respective type I or type II counterpart in the preexisting KRT1/KRT10 network or dimerize with another, as yet undiscovered keratin partner
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • CK 2e antibody
    • Cytokeratin-2e antibody
    • Epithelial keratin-2e antibody
    • K2e antibody
    • Keratin type II cytoskeletal 2 epidermal antibody
    • Keratin-2 epidermis antibody
    • KRT2 antibody
    • KRT2A antibody
    • KRT2E antibody
    • Type-II keratin Kb2 antibody
    see all

Images

  • Anti-Cytokeratin 2e antibody (ab197365) at 1/450 dilution + Jurkat cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 65 kDa


    Exposure time: 45 seconds


    10%SDS-PAGE.

  • Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling Cytokeratin 2e with ab197365 at 1/20 dilution.

References

ab197365 has not yet been referenced specifically in any publications.

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