Anti-Cytokeratin 2e antibody (ab231608)
Key features and details
- Rabbit polyclonal to Cytokeratin 2e
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Cytokeratin 2e antibody
See all Cytokeratin 2e primary antibodies -
Description
Rabbit polyclonal to Cytokeratin 2e -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Rat Cytokeratin 2e aa 500 to the C-terminus. N-terminal His-tag and S-tag. Expressed in E.coli.
Database link: Q6IG02 -
Positive control
- IHC-P: Rat skin tissue. WB: Rat liver and skin lysates; Rat serum; A431 cell lysate; Recombinant rat Cytokeratin 2e protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231608 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 69 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 69 kDa. |
Target
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Relevance
Keratins are the major gene product of keratinocytes and form the intermediate filament cytoskeletal network in these cells. In cells of the upper spinous layer, KRT2E and KRT9 are expressed. Although the expression of KRT9 is limited to palmoplantar epidermis, KRT2E is expressed not only in this tissue but also in other regions, notably the epidermis covering the knee, thigh, and groin. It is not known whether these keratins simply replace their respective type I or type II counterpart in the preexisting KRT1/KRT10 network or dimerize with another, as yet undiscovered keratin partner -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 3849 Human
- Entrez Gene: 406228 Rat
- Omim: 600194 Human
- SwissProt: P35908 Human
- SwissProt: Q6IG02 Rat
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Alternative names
- CK 2e antibody
- Cytokeratin-2e antibody
- Epithelial keratin-2e antibody
see all
Images
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Formalin-fixed, paraffin-embedded rat skin tissue stained for Cytokeratin 2e using ab231608 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-Cytokeratin 2e antibody (ab231608) at 2 µg/ml + Rat liver lysate
Predicted band size: 69 kDa -
Anti-Cytokeratin 2e antibody (ab231608) at 2 µg/ml + Rat serum
Predicted band size: 69 kDa -
Anti-Cytokeratin 2e antibody (ab231608) at 2 µg/ml + Rat skin lysate
Predicted band size: 69 kDa -
Anti-Cytokeratin 2e antibody (ab231608) at 2 µg/ml + A431 (human epidermoid carcinoma cell line) cell lysate
Predicted band size: 69 kDa -
Anti-Cytokeratin 2e antibody (ab231608) at 2 µg/ml + Recombinant rat Cytokeratin 2e protein
Predicted band size: 69 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231608 has not yet been referenced specifically in any publications.