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    cytokeratin-2e-antibody-ab231618.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Cytokeratin
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Anti-Cytokeratin 2e antibody (ab231618)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 2e antibody (ab231618)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 2e antibody (ab231618)
  • Western blot - Anti-Cytokeratin 2e antibody (ab231618)

Key features and details

  • Rabbit polyclonal to Cytokeratin 2e
  • Suitable for: IHC-P, WB
  • Reacts with: Rat, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Cytokeratin 2e antibody
    See all Cytokeratin 2e primary antibodies
  • Description

    Rabbit polyclonal to Cytokeratin 2e
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment (His-tag) corresponding to Rat Cytokeratin 2e aa 368-507. (Expressed in E.coli).
    Sequence:

    IAEVQSQYEIIAHKSKAESEELYHSKANEELQVTAVKHGDSLKEIKMEIS ELNRTIQRLQGEISHVKKQCKGVQDSIADAEQRGEHAIKDARGKLTDLEE ALQQGRENLARLLRDYQELMNVKLALDVEIATYRKLLEGE


    Database link: Q6IG02
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Recombinant rat Cytokeratin 2e protein. IHC-P: Rat cerebrum and skin tissues.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab231618 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Cytokeratin

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab231618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 20 µg/ml.
WB Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 69 kDa.

Target

  • Relevance

    Keratins are the major gene product of keratinocytes and form the intermediate filament cytoskeletal network in these cells. In cells of the upper spinous layer, KRT2E and KRT9 are expressed. Although the expression of KRT9 is limited to palmoplantar epidermis, KRT2E is expressed not only in this tissue but also in other regions, notably the epidermis covering the knee, thigh, and groin. It is not known whether these keratins simply replace their respective type I or type II counterpart in the preexisting KRT1/KRT10 network or dimerize with another, as yet undiscovered keratin partner
  • Cellular localization

    Cytoplasmic
  • Database links

    • Entrez Gene: 3849 Human
    • Entrez Gene: 16681 Mouse
    • Entrez Gene: 406228 Rat
    • Omim: 600194 Human
    • SwissProt: P35908 Human
    • SwissProt: Q3TTY5 Mouse
    • SwissProt: Q6IG02 Rat
    • Unigene: 707 Human
    • Unigene: 358616 Mouse
    see all
  • Alternative names

    • CK 2e antibody
    • Cytokeratin-2e antibody
    • Epithelial keratin-2e antibody
    • K2e antibody
    • Keratin type II cytoskeletal 2 epidermal antibody
    • Keratin-2 epidermis antibody
    • KRT2 antibody
    • KRT2A antibody
    • KRT2E antibody
    • Type-II keratin Kb2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 2e antibody (ab231618)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 2e antibody (ab231618)

    Formalin-fixed, paraffin-embedded rat skin tissue stained for Cytokeratin 2e using ab231618 at 20 µg/ml in immunohistochemical analysis. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 2e antibody (ab231618)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 2e antibody (ab231618)

    Paraffin-embedded rat cerebrum tissue stained for Cytokeratin 2e using ab231618 at 20 µg/ml in immunohistochemical analysis. DAB staining.

  • Western blot - Anti-Cytokeratin 2e antibody (ab231618)
    Western blot - Anti-Cytokeratin 2e antibody (ab231618)
    Anti-Cytokeratin 2e antibody (ab231618) at 2 µg/ml + Recombinant rat Cytokeratin 2e protein

    Predicted band size: 69 kDa

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab231618? Please let us know so that we can cite the reference in this datasheet.

    ab231618 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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