Overview

  • Product name
    Anti-Cytokeratin 2e antibody [EPR10911]
    See all Cytokeratin 2e primary antibodies
  • Description
    Rabbit monoclonal [EPR10911] to Cytokeratin 2e
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Protein fragment, corresponding to residues in Human Cytokeratin 2e (UniProt: P35908).

  • Positive control
    • A431 and HACAT lysates; Human skin tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10911
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 65 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Relevance
      Keratins are the major gene product of keratinocytes and form the intermediate filament cytoskeletal network in these cells. In cells of the upper spinous layer, KRT2E and KRT9 are expressed. Although the expression of KRT9 is limited to palmoplantar epidermis, KRT2E is expressed not only in this tissue but also in other regions, notably the epidermis covering the knee, thigh, and groin. It is not known whether these keratins simply replace their respective type I or type II counterpart in the preexisting KRT1/KRT10 network or dimerize with another, as yet undiscovered keratin partner
    • Cellular localization
      Cytoplasmic
    • Database links
    • Alternative names
      • CK 2e antibody
      • Cytokeratin-2e antibody
      • Epithelial keratin-2e antibody
      • K2e antibody
      • Keratin type II cytoskeletal 2 epidermal antibody
      • Keratin-2 epidermis antibody
      • KRT2 antibody
      • KRT2A antibody
      • KRT2E antibody
      • Type-II keratin Kb2 antibody
      see all

    Images

    • All lanes : Anti-Cytokeratin 2e antibody [EPR10911] (ab170106) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : HACAT cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 65 kDa

    • Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Cytokeratin 2e with ab170106 at a 1/100 dilution.

    References

    ab170106 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab170106.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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