Recombinant Anti-Cytokeratin 2e antibody [EPR10911] (ab170106)
Overview
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Product name
Anti-Cytokeratin 2e antibody [EPR10911]
See all Cytokeratin 2e primary antibodies -
Description
Rabbit monoclonal [EPR10911] to Cytokeratin 2e -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Cytokeratin 2e.
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Positive control
- A431 and HACAT lysates; Human skin tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10911 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab170106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 65 kDa. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Relevance
Keratins are the major gene product of keratinocytes and form the intermediate filament cytoskeletal network in these cells. In cells of the upper spinous layer, KRT2E and KRT9 are expressed. Although the expression of KRT9 is limited to palmoplantar epidermis, KRT2E is expressed not only in this tissue but also in other regions, notably the epidermis covering the knee, thigh, and groin. It is not known whether these keratins simply replace their respective type I or type II counterpart in the preexisting KRT1/KRT10 network or dimerize with another, as yet undiscovered keratin partner -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 3849 Human
- Omim: 600194 Human
- SwissProt: P35908 Human
- Unigene: 707 Human
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Alternative names
- CK 2e antibody
- Cytokeratin-2e antibody
- Epithelial keratin-2e antibody
see all
Images
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![Western blot - Anti-Cytokeratin 2e antibody [EPR10911] (ab170106)](https://www.abcam.com/ps/products/170/ab170106/Images/ab170106-170046-anti-cytokeratin-2e-antibody-epr10911-western-blot.jpg)
Western blot - Anti-Cytokeratin 2e antibody [EPR10911] (ab170106)All lanes : Anti-Cytokeratin 2e antibody [EPR10911] (ab170106) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HACAT cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 65 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 2e antibody [EPR10911] (ab170106)](https://www.abcam.com/ps/products/170/ab170106/Images/ab170106-170045-anti-cytokeratin-2e-antibody-epr10911-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 2e antibody [EPR10911] (ab170106)Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Cytokeratin 2e with ab170106 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References
ab170106 has not yet been referenced specifically in any publications.
