RecombinantRabMAb

Recombinant Anti-Cytokeratin 2e antibody [EPR10911] (ab170106)

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Western blot - Anti-Cytokeratin 2e antibody [EPR10911] (ab170106)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 2e antibody [EPR10911] (ab170106)
  • Anti-Cytokeratin 2e antibody [EPR10911] (ab170106)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10911] to Cytokeratin 2e
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Cytokeratin 2e antibody [EPR10911]
    See all Cytokeratin 2e primary antibodies

  • Description

    Rabbit monoclonal [EPR10911] to Cytokeratin 2e

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human Cytokeratin 2e.

  • Positive control

    • A431 and HACAT lysates; Human skin tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10911

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab170106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 65 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 65 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    • Relevance

      Keratins are the major gene product of keratinocytes and form the intermediate filament cytoskeletal network in these cells. In cells of the upper spinous layer, KRT2E and KRT9 are expressed. Although the expression of KRT9 is limited to palmoplantar epidermis, KRT2E is expressed not only in this tissue but also in other regions, notably the epidermis covering the knee, thigh, and groin. It is not known whether these keratins simply replace their respective type I or type II counterpart in the preexisting KRT1/KRT10 network or dimerize with another, as yet undiscovered keratin partner

    • Cellular localization

      Cytoplasmic

    • Database links

      • Alternative names

        • CK 2e antibody
        • Cytokeratin-2e antibody
        • Epithelial keratin-2e antibody
        • K2e antibody
        • Keratin type II cytoskeletal 2 epidermal antibody
        • Keratin-2 epidermis antibody
        • KRT2 antibody
        • KRT2A antibody
        • KRT2E antibody
        • Type-II keratin Kb2 antibody
        see all

      Images

      • Western blot - Anti-Cytokeratin 2e antibody [EPR10911] (ab170106)
        Western blot - Anti-Cytokeratin 2e antibody [EPR10911] (ab170106)
        All lanes : Anti-Cytokeratin 2e antibody [EPR10911] (ab170106) at 1/1000 dilution

        Lane 1 : A431 cell lysate
        Lane 2 : HACAT cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 65 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 2e antibody [EPR10911] (ab170106)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 2e antibody [EPR10911] (ab170106)

        Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Cytokeratin 2e with ab170106 at a 1/100 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Anti-Cytokeratin 2e antibody [EPR10911] (ab170106)
        Anti-Cytokeratin 2e antibody [EPR10911] (ab170106)

      References (0)

      Publishing research using ab170106? Please let us know so that we can cite the reference in this datasheet.

      ab170106 has not yet been referenced specifically in any publications.

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