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Rabbit monoclonal [EPR10911] to Cytokeratin 2e
IHC-P more details Unsuitable for:
Flow Cyt,ICC/IF or IP
Protein fragment, corresponding to residues in Human Cytokeratin 2e (UniProt: P35908).
A431 and HACAT lysates; Human skin tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 65 kDa.
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Is unsuitable for Flow Cyt,ICC/IF or IP.
Keratins are the major gene product of keratinocytes and form the intermediate filament cytoskeletal network in these cells. In cells of the upper spinous layer, KRT2E and KRT9 are expressed. Although the expression of KRT9 is limited to palmoplantar epidermis, KRT2E is expressed not only in this tissue but also in other regions, notably the epidermis covering the knee, thigh, and groin. It is not known whether these keratins simply replace their respective type I or type II counterpart in the preexisting KRT1/KRT10 network or dimerize with another, as yet undiscovered keratin partner
CK 2e antibody
Epithelial keratin-2e antibody
Western blot - Anti-Cytokeratin 2e antibody [EPR10911] (ab170106)
All lanes : Anti-Cytokeratin 2e antibody [EPR10911] (ab170106) at 1/1000 dilution Lane 1 : A431 cell lysate Lane 2 : HACAT cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat anti-rabbit HRP at 1/2000 dilution Predicted band size: 65 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 2e antibody [EPR10911] (ab170106)
Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Cytokeratin 2e with ab170106 at a 1/100 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"