Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab68260)
Key features and details
- Mouse monoclonal [AE5] to Cytokeratin 3/CK-3
- Reacts with: Mouse, Rabbit, Cow, Human
- Isotype: IgG1
Overview
-
Product name
Anti-Cytokeratin 3/CK-3 antibody [AE5]
See all Cytokeratin 3/CK-3 primary antibodies -
Description
Mouse monoclonal [AE5] to Cytokeratin 3/CK-3 -
Host species
Mouse -
Specificity
This antibody recognizes the 64kD polypeptide (cytokeratin 3 / CK-3) of rabbit corneal epithelium. It also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line. The clone number has been updated from (2Q1040) to (AE5) both clone numbers name the same antibody clone.
-
Species reactivity
Reacts with: Mouse, Rabbit, Cow, Human -
Immunogen
Tissue, cells or virus corresponding to Rabbit Cytokeratin 3/CK-3. Rabbit corneal epithelial keratin.
-
General notes
Previously labelled as Cytokeratin 3.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
AE5 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Target
-
Tissue specificity
Cornea specific. -
Involvement in disease
Defects in KRT3 are a cause of Meesmann corneal dystrophy (MECD) [MIM:122100]; also abbreviated as MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
-
Database links
- Entrez Gene: 281267 Cow
- Entrez Gene: 3850 Human
- Omim: 148043 Human
- SwissProt: P12035 Human
- SwissProt: Q29426 Rabbit
- Unigene: 680652 Human
-
Alternative names
- 65 kDa cytokeratin antibody
- CK-3 antibody
- CK3 antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (13)
ab68260 has been referenced in 13 publications.
- Garzón I et al. Long-Term in vivo Evaluation of Orthotypical and Heterotypical Bioengineered Human Corneas. Front Bioeng Biotechnol 8:681 (2020). PubMed: 32671048
- Menzel-Severing J et al. Transcription factor profiling identifies Sox9 as regulator of proliferation and differentiation in corneal epithelial stem/progenitor cells. Sci Rep 8:10268 (2018). PubMed: 29980721
- Xu W et al. Carboxymethyl chitosan/gelatin/hyaluronic acid blended-membranes as epithelia transplanting scaffold for corneal wound healing. Carbohydr Polym 192:240-250 (2018). PubMed: 29691018
- Lee HK et al. Protection of Corneal Limbus from Riboflavin Prevents Epithelial Stem Cell Loss after Collagen Cross-Linking. J Ophthalmol 2018:6854298 (2018). PubMed: 29967694
- Sun J et al. Differentiation of rat adipose-derived mesenchymal stem cells into corneal-like epithelial cells driven by PAX6. Exp Ther Med 15:1424-1432 (2018). PubMed: 29434727
