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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins
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Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab68260)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (13)

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Key features and details

  • Mouse monoclonal [AE5] to Cytokeratin 3/CK-3
  • Reacts with: Mouse, Rabbit, Cow, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Cytokeratin 3/CK-3 antibody [AE5]
    See all Cytokeratin 3/CK-3 primary antibodies
  • Description

    Mouse monoclonal [AE5] to Cytokeratin 3/CK-3
  • Host species

    Mouse
  • Specificity

    This antibody recognizes the 64kD polypeptide (cytokeratin 3 / CK-3) of rabbit corneal epithelium. It also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line. The clone number has been updated from (2Q1040) to (AE5) both clone numbers name the same antibody clone.

  • Species reactivity

    Reacts with: Mouse, Rabbit, Cow, Human
  • Immunogen

    Tissue, cells or virus corresponding to Rabbit Cytokeratin 3/CK-3. Rabbit corneal epithelial keratin.

  • General notes

    Previously labelled as Cytokeratin 3.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Ion Exchange Chromatography
  • Clonality

    Monoclonal
  • Clone number

    AE5
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins
    • Immunology
    • Immune System Diseases
    • Autoimmune
    • Neuroscience
    • Sensory System
    • Visual system

Associated products

  • Alternative Versions

    • Anti-Cytokeratin 3/CK-3 antibody [AE5] - BSA and Azide free (ab255962)
    • Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab77869)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Target

  • Tissue specificity

    Cornea specific.
  • Involvement in disease

    Defects in KRT3 are a cause of Meesmann corneal dystrophy (MECD) [MIM:122100]; also abbreviated as MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Target information above from: UniProt accession P12035 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 281267 Cow
    • Entrez Gene: 3850 Human
    • Omim: 148043 Human
    • SwissProt: P12035 Human
    • SwissProt: Q29426 Rabbit
    • Unigene: 680652 Human
    • Alternative names

      • 65 kDa cytokeratin antibody
      • CK-3 antibody
      • CK3 antibody
      • Cytokeratin 3 antibody
      • Cytokeratin-3 antibody
      • K2C3_HUMAN antibody
      • K3 antibody
      • Keratin 3 antibody
      • Keratin antibody
      • Keratin type II cytoskeletal 3 antibody
      • Keratin-3 antibody
      • KRT3 antibody
      • type II cytoskeletal 3 antibody
      • Type-II keratin Kb3 antibody
      see all

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (13)

    Publishing research using ab68260? Please let us know so that we can cite the reference in this datasheet.

    ab68260 has been referenced in 13 publications.

    • Garzón I  et al. Long-Term in vivo Evaluation of Orthotypical and Heterotypical Bioengineered Human Corneas. Front Bioeng Biotechnol 8:681 (2020). PubMed: 32671048
    • Menzel-Severing J  et al. Transcription factor profiling identifies Sox9 as regulator of proliferation and differentiation in corneal epithelial stem/progenitor cells. Sci Rep 8:10268 (2018). PubMed: 29980721
    • Xu W  et al. Carboxymethyl chitosan/gelatin/hyaluronic acid blended-membranes as epithelia transplanting scaffold for corneal wound healing. Carbohydr Polym 192:240-250 (2018). PubMed: 29691018
    • Lee HK  et al. Protection of Corneal Limbus from Riboflavin Prevents Epithelial Stem Cell Loss after Collagen Cross-Linking. J Ophthalmol 2018:6854298 (2018). PubMed: 29967694
    • Sun J  et al. Differentiation of rat adipose-derived mesenchymal stem cells into corneal-like epithelial cells driven by PAX6. Exp Ther Med 15:1424-1432 (2018). PubMed: 29434727
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 3/CK-3 antibody [AE5]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rabbit Tissue sections (Rabbit cornea)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer 10mM pH6
    Permeabilization
    Yes - 0.3% Triton X-100 for 5 minutes
    Specification
    Rabbit cornea
    Blocking step
    Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. Manuel Chacon

    Verified customer

    Submitted Jan 03 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 3/CK-3 antibody [AE5]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rabbit Cell (Cornea)
    Permeabilization
    Yes - 0.1% Triton X-100 in PBS
    Specification
    Cornea
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Aaron Gardner

    Verified customer

    Submitted Nov 20 2012

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