Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab68260)
- Datasheet
- References (9)
- Protocols
Overview
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Product name
Anti-Cytokeratin 3/CK-3 antibody [AE5]
See all Cytokeratin 3/CK-3 primary antibodies -
Description
Mouse monoclonal [AE5] to Cytokeratin 3/CK-3 -
Host species
Mouse -
Specificity
This antibody recognizes the 64kD polypeptide (cytokeratin 3 / CK-3) and 55 kDa polypeptide (cytokeratin 12) of rabbit corneal epithelium. It also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line. The clone number has been updated from (2Q1040) to (AE5) both clone numbers name the same antibody clone.
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Tested applications
Suitable for: ICC/IF, WB, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rabbit, Cow, Human -
Immunogen
Tissue, cells or virus corresponding to Rabbit Cytokeratin 3/CK-3. Rabbit corneal epithelial keratin.
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General notes
Previously labelled as Cytokeratin 3.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
AE5 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab68260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 54 , 65 kDa. | |
| IHC-P | 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IHC-Fr | 1/50. |
Target
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Tissue specificity
Cornea specific. -
Involvement in disease
Defects in KRT3 are a cause of Meesmann corneal dystrophy (MECD) [MIM:122100]; also abbreviated as MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 281267 Cow
- Entrez Gene: 3850 Human
- Omim: 148043 Human
- SwissProt: P12035 Human
- SwissProt: Q29426 Rabbit
- Unigene: 680652 Human
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Alternative names
- 65 kDa cytokeratin antibody
- CK-3 antibody
- CK3 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab68260)](https://www.abcam.com/ps/products/68/ab68260/Images/Cytokeratin-312-Primary-antibodies-ab68260-6.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab68260)This image is courtesy of an Abreview submitted by Dr Aaron GardnerImmunofluorescence analysis of rabbit cornea cells, staining Cytokeratin 3/CK-3+12 with ab68620.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/50 in 3% BSA in PBST (0.5% Tween 20)) for 12 hours at 4°C. An AlexaFluor®594-conjugated goat anti-mouse polyclonal IgG (1/100) was used as the secondary antibody. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab68260)](https://www.abcam.com/ps/products/68/ab68260/Images/ab68260-276267-ab68260-reference-ICC.PNG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab68260)Image cropped from Jing L et at., Molecular Vision, 17, 2263-2271, Fig. 2e Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.Immunofluorescent analysis of human ocular surface squamous cell carcinoma tissue, labeling Cytokeratin 3/CK-3+12 with ab68260. Samples were fixed in 4% paraformaldehyde and blocked wuth 4% BSA with 0.1% Triton X-100. Incubation with ab68260 (diluted 1/50) overnight at 4°C. Samples were then incubated with an Alexa Fluor 488-conjugated secondary antibody for 1 hour, and counterstained with DAPI to visualize nuclei.
Protocols
References
This product has been referenced in:
- Xu W et al. Carboxymethyl chitosan/gelatin/hyaluronic acid blended-membranes as epithelia transplanting scaffold for corneal wound healing. Carbohydr Polym 192:240-250 (2018). Read more (PubMed: 29691018) »
- Lee HK et al. Protection of Corneal Limbus from Riboflavin Prevents Epithelial Stem Cell Loss after Collagen Cross-Linking. J Ophthalmol 2018:6854298 (2018). Read more (PubMed: 29967694) »
