• Product name

    Anti-Cytokeratin 4 antibody [6B10]
    See all Cytokeratin 4 primary antibodies
  • Description

    Mouse monoclonal [6B10] to Cytokeratin 4
  • Host species

  • Specificity

    Reacts exclusively with cytokeratin 4 which is present in non-cornifying squamous epithelium, including cornea and transitional epithelium. Cells in certain ciliated pseudo-stratified epithelia and ductal epithelia of various exocrine glands are also positive. Normally keratin 4 is not present in the layers of the epidermis, but should be detectable in glandular tissue of the skin (sweat glands). Skin epidermis contains mainly cytokeratins 14 and 19 (in the basal layer) and cytokeratin 1 and 10 in the cornifying layers. Cytokeratin 4 has a molecular weight of approximately 59 kDa.
  • Tested applications

    Suitable for: IHC-P, ICC, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Cat, Dog, Human, Zebrafish
  • Immunogen

    Fusion protein corresponding to Cytokeratin 4. ab9004 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a Cytokeratin preparation extracted from Human esophagus.
    Database link: P19013



Our Abpromise guarantee covers the use of ab9004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 20036603
ICC Use at an assay dependent concentration.
WB 1/100 - 1/1000. Predicted molecular weight: 57 kDa.
Flow Cyt 1/25 - 1/200.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • Tissue specificity

    Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer.
  • Involvement in disease

    Defects in KRT4 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Information by UniProt
  • Database links

  • Form

    Localisation: Intermediate filament (Cytoskeleton).
  • Alternative names

    • CK 4 antibody
    • CK-4 antibody
    • CK4 antibody
    • CYK4 antibody
    • Cytokeratin 4 antibody
    • Cytokeratin-4 antibody
    • Cytokeratin4 antibody
    • FLJ31692 antibody
    • K2C4_HUMAN antibody
    • K4 antibody
    • Keratin 4 antibody
    • Keratin antibody
    • Keratin type II cytoskeletal 4 antibody
    • Keratin-4 antibody
    • Keratin4 antibody
    • KRT 4 antibody
    • Krt4 antibody
    • type II cytoskeletal 4 antibody
    • Type-II keratin Kb4 antibody
    see all


  • ab9004 at a 1/100 dilution staining Cytokeratin 4 in human leukoplakia lesions by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Top image: Normal tissue.
    Middle image: Tumor tissue.
    Bottom image: Leukoplakia non-progressing.

    Paraffin sections were deparaffinized, re-hydrated, subjected to antigen retrieval by microwave boiling for 10 minutes in 10 mM TRIS pH 9.0, 1 mM EDTA, and pre-incubated for 15 minutes with 2% normal rabbit serum. The second step was performed with a biotinylated rabbit anti-mouse at a 1/500 dilution and in the final step horseradish peroxidase labeled streptavidin–biotincomplex was applied. The staining was developed with diaminobenzidine and H2O2 as chromogen. The sections were counterstained with haematoxylin and coverslipped with Kaiser’s glycerin.


This product has been referenced in:

  • Jolly AL  et al. Stromal Fibroblasts Drive Host Inflammatory Responses That Are Dependent on Chlamydia trachomatis Strain Type and Likely Influence Disease Outcomes. MBio 10:N/A (2019). Read more (PubMed: 30890604) »
  • Badylak SF  et al. Esophageal preservation in five male patients after endoscopic inner-layer circumferential resection in the setting of superficial cancer: a regenerative medicine approach with a biologic scaffold. Tissue Eng Part A 17:1643-50 (2011). IHC-P ; Human . Read more (PubMed: 21306292) »
See all 7 Publications for this product

Customer reviews and Q&As

1-3 of 3 Q&A


Thank you for contacting us about this issue.

You should detect cytokeratin 4 at about 59kDa, assuming your samples are reduced and denatured. Can you please tell me what your samples are, and how they are prepared? Could you also send a few details of your blot protocol? In particular, we are interested in the amount of sample you loaded per lane of the gel, whether transfer was checked with a stain before incubating with the antibody, how the membrane was blocked, and how much antibody you incubated with the membrane (i.e., the dilution or concentration of the antibody).

I will also need the approximate date of your order.

I look forward to your reply.

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Thank you for contacting Abcam regarding ab9004.

I can confirm that [6B10] in the product name refers to the clone number and is not at all indicative of any modifications to cytokeratin 4.

I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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I apologize for the delay.

Vimentin is a mesenchymal cell marker, ab8978 is tested in Zebrafish. It might be suitable for your purpose.

ab9004, ab9021, ab9217 are anti Cytokeratin 4,7 and 18. Against epithelial cell marker the antibodies is tested in Zebrafish.

Could you more specific to type of blood cell marker you are interested in?

We do not have anti M-Cadherin and Myo-D antibodies that could be used for Zebrafish. This assumption is based on the protein sequence similarity between human and Zebrafish. We unfortunately do not have antibodies against these target that might cross react.

I have attached a Excel file of all Zebrafish specific antibodies with immunogen sequence. This file will help you selecting the antibodies based on cross reactivity due to immunogen sequence homology.

I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.

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