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We purchased the anti-cytokeratin 4 antibody (ab9004) and did western blot. However, it did not show any band at 59 kDa, but got clear 3 non specific bands at about 150 kDa area, do you know whether we should ignore that? Or that also mean something?
Asked on Sep 13 2012
Thank you for contacting us about this issue.
You should detect cytokeratin 4 at about 59kDa, assuming your samples are reduced and denatured. Can you please tell me what your samples are, and how they are prepared? Could you also send a few details of your blot protocol? In particular, we are interested in the amount of sample you loaded per lane of the gel, whether transfer was checked with a stain before incubating with the antibody, how the membrane was blocked, and how much antibody you incubated with the membrane (i.e., the dilution or concentration of the antibody).
I will also need the approximate date of your order.
I look forward to your reply.
Answered on Sep 13 2012