- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Cytokeratin 4 antibody
See all Cytokeratin 4 primary antibodies -
DescriptionRabbit polyclonal to Cytokeratin 4
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 317-505 of Human Cytokeratin 4.
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Positive control
- HeLa whole cell lysate; HeLa cells; Human BT474 xenograft tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab154406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 57 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Tissue specificityDetected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer.
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Involvement in diseaseDefects in KRT4 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
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Sequence similaritiesBelongs to the intermediate filament family.
- Information by UniProt
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Database links
- Entrez Gene: 3851 Human
- Omim: 123940 Human
- SwissProt: P19013 Human
- Unigene: 654610 Human
- Unigene: 706527 Human
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FormLocalisation: Intermediate filament (Cytoskeleton).
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Alternative names
- CK 4 antibody
- CK-4 antibody
- CK4 antibody
see all
Images
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Western blot - Anti-Cytokeratin 4 antibody (ab154406)Anti-Cytokeratin 4 antibody (ab154406) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 57 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 4 antibody (ab154406)Immunohistochemical analysis of paraffin-embedded Human BT474 xenograft tissue, labeling Cytokeratin 4 with ab154406 at 1/500 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 4 antibody (ab154406)Immunofluorescence analysis of methanol-fixed HeLa cells (iced-cold, 5 min) labeling Cytokeratin 4 with ab154406 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.
Protocols
References
ab154406 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"