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    cytokeratin-4-antibody-ab154406.pdf

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Tags & Cell Markers Cell Type Markers Epi / Endo-thelial
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Anti-Cytokeratin 4 antibody (ab154406)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 4 antibody (ab154406)
  • Western blot - Anti-Cytokeratin 4 antibody (ab154406)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 4 antibody (ab154406)

Key features and details

  • Rabbit polyclonal to Cytokeratin 4
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Cytokeratin 4 antibody
    See all Cytokeratin 4 primary antibodies
  • Description

    Rabbit polyclonal to Cytokeratin 4
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 317-505 of Human Cytokeratin 4.

  • Positive control

    • HeLa whole cell lysate; HeLa cells; Human BT474 xenograft tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Epi / Endo-thelial
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Cytokeratin

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.

Target

  • Tissue specificity

    Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer.
  • Involvement in disease

    Defects in KRT4 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Target information above from: UniProt accession P19013 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3851 Human
    • Omim: 123940 Human
    • SwissProt: P19013 Human
    • Unigene: 654610 Human
    • Unigene: 706527 Human
    • Form

      Localisation: Intermediate filament (Cytoskeleton).
    • Alternative names

      • CK 4 antibody
      • CK-4 antibody
      • CK4 antibody
      • CYK4 antibody
      • Cytokeratin 4 antibody
      • Cytokeratin-4 antibody
      • Cytokeratin4 antibody
      • FLJ31692 antibody
      • K2C4_HUMAN antibody
      • K4 antibody
      • Keratin 4 antibody
      • Keratin antibody
      • Keratin type II cytoskeletal 4 antibody
      • Keratin-4 antibody
      • Keratin4 antibody
      • KRT 4 antibody
      • Krt4 antibody
      • type II cytoskeletal 4 antibody
      • Type-II keratin Kb4 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 4 antibody (ab154406)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 4 antibody (ab154406)
      Immunohistochemical analysis of paraffin-embedded Human BT474 xenograft tissue, labeling Cytokeratin 4 with ab154406 at 1/500 dilution.
    • Western blot - Anti-Cytokeratin 4 antibody (ab154406)
      Western blot - Anti-Cytokeratin 4 antibody (ab154406)
      Anti-Cytokeratin 4 antibody (ab154406) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

      Predicted band size: 57 kDa



      7.5% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 4 antibody (ab154406)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 4 antibody (ab154406)
      Immunofluorescence analysis of methanol-fixed HeLa cells (iced-cold, 5 min) labeling Cytokeratin 4 with ab154406 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab154406? Please let us know so that we can cite the reference in this datasheet.

    ab154406 has not yet been referenced specifically in any publications.

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