Anti-Cytokeratin 4 antibody [EP1599Y] (ab51599)
- Datasheet
- Specific References (7)
- Protocols
Overview
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Product nameAnti-Cytokeratin 4 antibody [EP1599Y]
See all Cytokeratin 4 primary antibodies -
DescriptionRabbit monoclonal [EP1599Y] to Cytokeratin 4
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IP, WB, IHC-P, Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human Cytokeratin 4 (C terminal). The exact sequence is proprietary.
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Positive control
- A431 cell lysate and human cervical carcinoma tissue. This antibody gave a positive result when used in the following methanol fixed cell lines: A431
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEP1599Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab51599 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/50 - 1/100. | |
| IP | 1/10 - 1/100. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 57 kDa). | |
| IHC-P | 1/100 - 1/250. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Tissue specificityDetected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer.
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Involvement in diseaseDefects in KRT4 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
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Sequence similaritiesBelongs to the intermediate filament family.
- Information by UniProt
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Database links
- Entrez Gene: 3851 Human
- Omim: 123940 Human
- SwissProt: P19013 Human
- Unigene: 654610 Human
- Unigene: 706527 Human
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FormLocalisation: Intermediate filament (Cytoskeleton).
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Alternative names
- CK 4 antibody
- CK-4 antibody
- CK4 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 4 antibody [EP1599Y] (ab51599)](https://www.abcam.com/ps/products/51/ab51599/Images/ab51599-219237-ab51599gr962534edited.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 4 antibody [EP1599Y] (ab51599)ICC/IF image of ab51599 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab51599 at 1/100 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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![Western blot - Anti-Cytokeratin 4 antibody [EP1599Y] (ab51599)](https://www.abcam.com/ps/products/51/ab51599/Images/ab51599 wb.bmp)
Western blot - Anti-Cytokeratin 4 antibody [EP1599Y] (ab51599)Anti-Cytokeratin 4 antibody [EP1599Y] (ab51599) at 1/1000 dilution + A431 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 57 kDa
Observed band size: 59 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 4 antibody [EP1599Y] (ab51599)](https://www.abcam.com/ps/products/51/ab51599/Images/ab51599_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 4 antibody [EP1599Y] (ab51599)Immunohistochemical staining of paraffin embedded human cervical carcinoma tissue using ab51599 (1/100). Goat anti-rabbit HRP (1/2000) used for secondary staining.
References
This product has been referenced in:
- Liu S et al. Tyrosine kinase LYN is an oncotarget in human cervical cancer: A quantitative proteomic based study. Oncotarget 7:75468-75481 (2016). WB ; Human . Read more (PubMed: 27690342) »
- Lehman HL et al. p120-catenin down-regulation and epidermal growth factor receptor overexpression results in a transformed epithelium that mimics esophageal squamous cell carcinoma. Am J Pathol 185:240-51 (2015). Read more (PubMed: 25529795) »
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