• Product name

    Anti-Cytokeratin 4 antibody [EPR1598]
    See all Cytokeratin 4 primary antibodies
  • Description

    Rabbit monoclonal [EPR1598] to Cytokeratin 4
  • Host species

  • Specificity

    The immunogen shares high homology with other cytokeratins like cytokeratin-3(93%), cytokeratin-79(85.7%) and cytokeratin-2 oral(85.7%) etc.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Cytokeratin 4.

  • Positive control

    • WB: A431 and Human uterus cell lysates IHC-P: Human tonsil tissue ICC/IF: A431 cells
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab92465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ICC/IF: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. Antigen retrieval is recommended before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
    WB: 1/1000 - 1/10,000. Predicted molecular weight: 57 kDa.

    Is unsuitable for Flow Cyt or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Tissue specificity

      Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer.
    • Involvement in disease

      Defects in KRT4 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
    • Sequence similarities

      Belongs to the intermediate filament family.
    • Information by UniProt
    • Database links

    • Form

      Localisation: Intermediate filament (Cytoskeleton).
    • Alternative names

      • CK 4 antibody
      • CK-4 antibody
      • CK4 antibody
      • CYK4 antibody
      • Cytokeratin 4 antibody
      • Cytokeratin-4 antibody
      • Cytokeratin4 antibody
      • FLJ31692 antibody
      • K2C4_HUMAN antibody
      • K4 antibody
      • Keratin 4 antibody
      • Keratin antibody
      • Keratin type II cytoskeletal 4 antibody
      • Keratin-4 antibody
      • Keratin4 antibody
      • KRT 4 antibody
      • Krt4 antibody
      • type II cytoskeletal 4 antibody
      • Type-II keratin Kb4 antibody
      see all


    • ab92465, at a 1/100 dilution, staining Cytokeratin 4 in paraffin embedded Human tonsil tissue by Immunohistochemistry. Note positive staining of squamous cells.
    • All lanes : Anti-Cytokeratin 4 antibody [EPR1598] (ab92465) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : Human uterus cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 57 kDa

    • Immunofluorescent staining of A431 cells, using ab92465 at a 1/100 dilution.


    This product has been referenced in:

    • Jerg A  et al. Modulation of gingival cell response towards dental composites. Dent Mater 34:412-426 (2018). Read more (PubMed: 29306486) »
    • Jacot TA  et al. Development of a composite measure of product adherence, protocol compliance, and semen exposure using DNA and protein biomarkers for topical HIV prevention studies. PLoS One 9:e114368 (2014). ICC ; Human . Read more (PubMed: 25489736) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab92465.
    Please use the links above to contact us or submit feedback about this product.

    For licensing inquiries, please contact partnerships@abcam.com

    Sign up