Product nameAnti-Cytokeratin 5 + 14 antibody [16.4]
See all Cytokeratin 5 + 14 primary antibodies
DescriptionMouse monoclonal [16.4] to Cytokeratin 5 + 14
Tested applicationsSuitable for: ICC/IF, IHC-Fr, WBmore details
Species reactivityReacts with: Human
Tissue/ cell preparation (cytokeratin enriched extract of feline tongue epithelium).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.08% Sodium azide
Concentration information loading...
PurityProtein A/G purified
Our Abpromise guarantee covers the use of ab16570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 58 and 50 kDa corresponding to cytokeratins 5 and 14 respectively.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceCytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (i.e. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells.
Cellular localizationCytoplasmic; Basal layer
- CK 14 antibody
- CK 5 antibody
- DDD antibody
This product has been referenced in:
- Arthur GK et al. KCa3.1 K+ Channel Expression and Function in Human Bronchial Epithelial Cells. PLoS One 10:e0145259 (2015). Read more (PubMed: 26689552) »
- Woodman LB et al. Synthetic response of stimulated respiratory epithelium: modulation by prednisolone and iKK2 inhibition. Chest 143:1656-66 (2013). ICC/IF ; Human . Read more (PubMed: 23238614) »