Anti-Cytokeratin 5 + 8 antibody [RCK102] (ab9005)
Key features and details
- Mouse monoclonal [RCK102] to Cytokeratin 5 + 8
- Suitable for: Flow Cyt, IHC-Fr, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Cytokeratin 5 + 8 antibody [RCK102] -
Description
Mouse monoclonal [RCK102] to Cytokeratin 5 + 8 -
Host species
Mouse -
Specificity
This antibody reacts with cytokeratin 5 and cytokeratin 8, recognizing virtually all epithelial tissues and carcinomas. -
Tested applications
Suitable for: Flow Cyt, IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human Cytokeratin 5 + 8. Human lung cancer cell line (MR21).
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General notes
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. -
Clonality
Monoclonal -
Clone number
RCK102 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/100 - 1/200.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt
1/100 - 1/200. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Cytokeratin 5 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. -
Cellular localization
Cell Membrane and Cytoplasmic -
Database links
- Entrez Gene: 3852 Human
- Entrez Gene: 3856 Human
- Omim: 148040 Human
- Omim: 148060 Human
- SwissProt: P05787 Human
- SwissProt: P13647 Human
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Alternative names
- CK5 antibody
- CK8 antibody
- CYK 8 antibody
see all
Images
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IHC image of ab9005 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9005, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Overlay histogram showing A431 cells stained with ab9005 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9005, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.
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Immunohistochemistry analysis on frozen sections of human liver hepatocytes labeling Cytokeratin 5 + 8 using ab9005.
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Immunohistochemistry analysis on frozen sections of human liver hepatocytes and bile ducts labeling Cytokeratin 5 + 8 using ab9005.
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Immunohistochemistry analysis on frozen sections of human urinary bladder epithelium labeling Cytokeratin 5 + 8 using ab9005.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab9005 has been referenced in 9 publications.
- Walker WH et al. Circadian Influences on Chemotherapy Efficacy in a Mouse Model of Brain Metastases of Breast Cancer. Front Oncol 11:752331 (2021). PubMed: 34956876
- Halim NS et al. The effect of mesenchymal stem cell-secreted factors on airway epithelial repair. Regen Med N/A:N/A (2018). PubMed: 30566028
- Kardia E et al. Stimulatory Secretions of Airway Epithelial Cells Accelerate Early Repair of Tracheal Epithelium. Sci Rep 7:11732 (2017). PubMed: 28916766
- Sathyanesan M et al. A molecular characterization of the choroid plexus and stress-induced gene regulation. Transl Psychiatry 2:e139 (2012). IHC-Fr ; Rat . PubMed: 22781172
- DeKruyff RH et al. T cell/transmembrane, Ig, and mucin-3 allelic variants differentially recognize phosphatidylserine and mediate phagocytosis of apoptotic cells. J Immunol 184:1918-30 (2010). PubMed: 20083673
- Skibinski G et al. Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor. Am J Physiol Lung Cell Mol Physiol 293:L69-76 (2007). PubMed: 17384084
- Smedts F et al. Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am J Pathol 136:657-68 (1990). PubMed: 1690513
- Ramaekers F et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 136:641-55 (1990). PubMed: 1690512
- Schaafsma HE et al. Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression. Am J Pathol 136:329-43 (1990). PubMed: 1689541