Overview

  • Product name
    Anti-Cytokeratin 5 + 8 antibody [RCK102]
  • Description
    Mouse monoclonal [RCK102] to Cytokeratin 5 + 8
  • Host species
    Mouse
  • Specificity
    This antibody reacts with cytokeratin 5 and cytokeratin 8, recognizing virtually all epithelial tissues and carcinomas.
  • Tested applications
    Suitable for: ICC/IF, Dot blot, Flow Cyt, IHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Hamster, Dog, Human, Pig
  • Immunogen

    Human lung cancer cell line (MR21).

  • General notes


    Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
  • Clonality
    Monoclonal
  • Clone number
    RCK102
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Dot blot 1/100 - 1/1000.
Flow Cyt 1/100 - 1/200.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/1000.

Target

  • Relevance
    Cytokeratin 5 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
  • Cellular localization
    Cell Membrane and Cytoplasmic
  • Database links
  • Alternative names
    • CK5 antibody
    • CK8 antibody
    • CYK 8 antibody
    • CYK8 antibody
    • Cytokeratin 5 antibody
    • Cytokeratin 8 antibody
    • K5 antibody
    • K8 antibody
    • Keratin 5 antibody
    • Keratin 8 antibody
    • Keratin type II cytoskeletal 5 antibody
    • Keratin type II cytoskeletal 8 antibody
    • KRT5 antibody
    • KRT8 antibody
    see all

Images

  • IHC image of ab9005 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9005, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing A431 cells stained with ab9005 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9005, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

  • Immunohistochemistry analysis on frozen sections of human liver hepatocytes labeling Cytokeratin 5 + 8 using ab9005.

  • Immunohistochemistry analysis on frozen sections of human liver hepatocytes and bile ducts labeling Cytokeratin 5 + 8 using ab9005.

  • Immunohistochemistry analysis on frozen sections of human urinary bladder epithelium labeling Cytokeratin 5 + 8 using ab9005.

References

This product has been referenced in:
  • Kardia E  et al. Stimulatory Secretions of Airway Epithelial Cells Accelerate Early Repair of Tracheal Epithelium. Sci Rep 7:11732 (2017). Read more (PubMed: 28916766) »
  • Sathyanesan M  et al. A molecular characterization of the choroid plexus and stress-induced gene regulation. Transl Psychiatry 2:e139 (2012). IHC-Fr ; Rat . Read more (PubMed: 22781172) »
See all 6 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (Primary airway epithelial cells)
Permeabilization
No
Specification
Primary airway epithelial cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
95% ethanol

Dr. Egi Kardia

Verified customer

Submitted Sep 10 2018

Answer

Thank you for contacting us.

The epithelial monolayer should be fine to you.

The Abpromise guarantee comes with all the tested applications and species mentioned on the datasheet. In that case theproducts are not eligible for Abreview discount codes i.e. if they are already tested. Discount codes are only provided for untested applications and species if the immunogenic homology is more than 84%.

DISCOUNT CODE: XXXXXXXX
Expiration date: 18-05-2012

I am very pleased to hear you would like to accept our offer and test ab90102 in Rabbit. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer, please submit an Abreview for Rabbit and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for contacting us.

The Cytokeratin 5 is about 84% similar between human and rabbit so ab9005 can also cross react. This antibody is also eligible for discount code offer.

At Abcam we have started using ICC/IF (Immunocytochemistry/ Immunofluorescence) instead of ICC or IF. However there are few old products that still shows ICC and they need to be updated. I have updated the datasheets now which will appear online soon. I apologize for the confusion this info have caused.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting us.
As discussed yesterday I can confirm that these antibodies can be used in ICC/IF.
The ab90102 is most likely to cross react with rabbit protein also. The sequence homology of human Cytokeratin 8 with Rabbit protein is 89% and for Cytokeratin it is 88%. Based on this sequence homology I am presuming that the antibody can cross react.
Rabbit currently is an untested species for this antibody so we are unable to guarantee it as tested species. Therefore, I can offer a discount off a future purchase if you buy ab90102 now, use it Rabbit and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free [PRIMARY ANTIBODY].
It doesn’t matter whether the result is positive or negative, we would just really like to receive your feedback. Of course, if the results are negative you will be covered by our Abpromise guarantee and eligible for a refund or replacement.
If you are interested in this offer, please follow these steps:
1. Reply to this e-mail to let me know that you would like to proceed and send an image of ab90102 in [Rabbit]. I will then send a discount code. This code must be issued before purchasing ab90102 so please wait for my reply before ordering.
2. Purchase ab90102 either by phone, fax, or online (www.abcam.com).
3. Use it in Rabbit.
4. Send us an image of the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any [PRIMARY ANTIBODY] ordered and the discount code is valid for 4 months after issue.
We are always pleased to obtain feedback about our products and any information is greatly appreciated!
The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.
Please let me know if you have any questions about this offer and I would be happy to help you further.

Read More

Answer

There is a very high chance that this antibody will recognise melanocytes. However, being as it has not been specifically tested on melanocytes we can't be certain

Read More

Question
Answer

Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we offer a replacement or reimbursement. We do not routinely offer free samples. We also offer a USD20 Amazon gift voucher to researchers who feedback information about our products.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up