Key features and details
- Mouse monoclonal [10C11E6] to Cytokeratin 5
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Cytokeratin 5 antibody [10C11E6]
See all Cytokeratin 5 primary antibodies
DescriptionMouse monoclonal [10C11E6] to Cytokeratin 5
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Chimpanzee
Recombinant fragment corresponding to Human Cytokeratin 5 aa 316-590. (Expressed in E.coli).
THVSDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIANRSRTEAESWYQTKY EELQQTAGRHGDDLRNTKHEISEMNRMIQRLRAEIDNVKKQCANLQNAIA DAEQRGELALKDARNKLAELEEALQKAKQDMARLLREYQELMNTKLALDV EIATYRKLLEGEECRLSGEGVGPVNISVVTSSVSSGYGSGSGYGGGLGGG LGGGLGGGLAGGSSGSYYSSSSGGVGLGGGLSVGGSGFSA SSGRGLGVGFGSGGGSSSSVKFVSTTSSSRKSFKS
Database link: P13647
- Human Cytokeratin 5 recombinant protein; A431 cell lysate; Human bladder cancer and esophagus cancer tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Contains 0.5% protein stabilizer.
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab181491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/1000.|
|WB||1/500 - 1/2000. Predicted molecular weight: 62 kDa.|
Involvement in diseaseDefects in KRT5 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
Defects in KRT5 are the cause of epidermolysis bullosa simplex with migratory circinate erythema (EBSMCE) [MIM:609352]. EBSMCE is a form of intraepidermal epidermolysis bullosa characterized by unusual migratory circinate erythema. Skin lesions appear from birth primarily on the hands, feet, and legs but spare nails, ocular epithelia and mucosae. Lesions heal with brown pigmentation but no scarring. Electron microscopy findings are distinct from those seen in the DM-EBS, with no evidence of tonofilament clumping.
Defects in KRT5 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
Defects in KRT5 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, althought it is less severe.
Defects in KRT5 are the cause of epidermolysis bullosa simplex with mottled pigmentation (MP-EBS) [MIM:131960]. MP-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering at acral sites and 'mottled' pigmentation of the trunk and proximal extremities with hyper- and hypopigmentation macules.
Defects in KRT5 are the cause of Dowling-Degos disease (DDD) [MIM:179850]; also known as Dowling-Degos-Kitamura disease or reticulate acropigmentation of Kitamura. DDD is an autosomal dominant genodermatosis. Affected individuals develop a postpubertal reticulate hyperpigmentation that is progressive and disfiguring, and small hyperkeratotic dark brown papules that affect mainly the flexures and great skin folds. Patients usually show no abnormalities of the hair or nails.
Sequence similaritiesBelongs to the intermediate filament family.
- Information by UniProt
- 58 kDa cytokeratin antibody
- CK-5 antibody
- CK5 antibody
Anti-Cytokeratin 5 antibody [10C11E6] (ab181491) at 1/500 dilution + A431 cell lysate
Predicted band size: 62 kDa
Anti-Cytokeratin 5 antibody [10C11E6] (ab181491) at 1/500 dilution + Human Cytokeratin 5 recombinant protein.
Predicted band size: 62 kDa
Expected MWt is 47.8 kDa.
Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue labeling Cytokeratin 5 with ab181491 at 1/200 dilution, with DAB staining.
Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling Cytokeratin 5 with ab181491 at 1/200 dilution, with DAB staining.
ab181491 has been referenced in 1 publication.
- Toor A et al. Transcriptomic and proteomic host response to Aspergillus fumigatus conidia in an air-liquid interface model of human bronchial epithelium. PLoS One 13:e0209652 (2018). PubMed: 30589860