Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 5 antibody

Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Ureter)
Specification
Ureter
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citric Acid Buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 21°C

Other product details

Dilution
1/250
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS, 0.1%BSA

Secondary antibody

Name
Non-Abcam antibody was used: Dako biotinylated goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/800

Additional data

Additional Notes
Strong reactivity with pig urothelial cells. Cross-reactivity with other porcine cytokeratins is likely, as labelling was observed across all urothelial cell layers.
10% swine serum with secondary antibody.

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Submitted Jun 02 2011

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