Overview

  • Product name
    Anti-Cytokeratin 5 antibody [SP27]
    See all Cytokeratin 5 primary antibodies
  • Description
    Rabbit monoclonal [SP27] to Cytokeratin 5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide within Human Cytokeratin 5 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P13647

  • Positive control
    • WB: A431 whole cell lysate. IHC-P: Human prostate adenocarcinoma, prostate, thymus, skin, pancreatic adenocarcinoma, lung, lung squamous cell carcinoma, breast, cervical squamous cell carcinoma, bladder and bladder transtitional cell carcinoma tissue. FC: A431 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.50
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purification notes
    Purified from TCS by protein A/G.
  • Clonality
    Monoclonal
  • Clone number
    SP27
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Predicted molecular weight: 62 kDa.
Flow Cyt 1/100.
IHC-P 1/100. Incubate for 30 min at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

Target

  • Involvement in disease
    Defects in KRT5 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
    Defects in KRT5 are the cause of epidermolysis bullosa simplex with migratory circinate erythema (EBSMCE) [MIM:609352]. EBSMCE is a form of intraepidermal epidermolysis bullosa characterized by unusual migratory circinate erythema. Skin lesions appear from birth primarily on the hands, feet, and legs but spare nails, ocular epithelia and mucosae. Lesions heal with brown pigmentation but no scarring. Electron microscopy findings are distinct from those seen in the DM-EBS, with no evidence of tonofilament clumping.
    Defects in KRT5 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
    Defects in KRT5 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, althought it is less severe.
    Defects in KRT5 are the cause of epidermolysis bullosa simplex with mottled pigmentation (MP-EBS) [MIM:131960]. MP-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering at acral sites and 'mottled' pigmentation of the trunk and proximal extremities with hyper- and hypopigmentation macules.
    Defects in KRT5 are the cause of Dowling-Degos disease (DDD) [MIM:179850]; also known as Dowling-Degos-Kitamura disease or reticulate acropigmentation of Kitamura. DDD is an autosomal dominant genodermatosis. Affected individuals develop a postpubertal reticulate hyperpigmentation that is progressive and disfiguring, and small hyperkeratotic dark brown papules that affect mainly the flexures and great skin folds. Patients usually show no abnormalities of the hair or nails.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 58 kDa cytokeratin antibody
    • CK-5 antibody
    • CK5 antibody
    • Cytokeratin-5 antibody
    • Cytokeratin5 antibody
    • DDD antibody
    • DDD1 antibody
    • EBS2 antibody
    • epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody
    • K2C5_HUMAN antibody
    • K5 antibody
    • keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) antibody
    • Keratin 5 antibody
    • Keratin antibody
    • keratin complex 2, basic, gene 5 antibody
    • keratin, type II cytoskeletal 5 antibody
    • Keratin-5 antibody
    • Keratin5 antibody
    • KRT 5 antibody
    • Krt5 antibody
    • KRT5A antibody
    • type II cytoskeletal 5 antibody
    • Type-II keratin Kb5 antibody
    see all

Images

  • Anti-Cytokeratin 5 antibody [SP27] (ab64081) at 1/100 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate

    Predicted band size: 62 kDa
    Observed band size: 53 kDa (why is the actual band size different from the predicted?)

  • Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

  • Flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cell line labeling Cytokeratin 5 with ab64081 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).

  • Formalin-fixed, paraffin-embedded human prostate tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human thymus tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human skin tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human breast tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human bladder tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

References

This product has been referenced in:
  • Hustler A  et al. Differential transcription factor expression by human epithelial cells of buccal and urothelial derivation. Exp Cell Res N/A:N/A (2018). IHC ; Human . Read more (PubMed: 29842880) »
  • Kalinsky K  et al. Proteomic modulation in breast tumors after metformin exposure: results from a "window of opportunity" trial. Clin Transl Oncol 19:180-188 (2017). Read more (PubMed: 27305912) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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