• Product name
    Anti-Cytokeratin 5 antibody [XM26]
    See all Cytokeratin 5 primary antibodies
  • Description
    Mouse monoclonal [XM26] to Cytokeratin 5
  • Host species
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, Flow Cyt, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Prokaryotic recombinant protein corresponding to 103 amino acid portion of the C-terminal region of the cytokeratin 5 molecule (Human).

  • Positive control
    • Skin


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: Tissue culture supernatant, 1% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
  • Clone number
  • Isotype
  • Light chain type
  • Research areas


Our Abpromise guarantee covers the use of ab17130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/75. An incubation period of 30 minutes at room temperature is recommended. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
IHC-Fr Use at an assay dependent concentration. PMID: 17065488
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
ICC 1/100.


  • Involvement in disease
    Defects in KRT5 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
    Defects in KRT5 are the cause of epidermolysis bullosa simplex with migratory circinate erythema (EBSMCE) [MIM:609352]. EBSMCE is a form of intraepidermal epidermolysis bullosa characterized by unusual migratory circinate erythema. Skin lesions appear from birth primarily on the hands, feet, and legs but spare nails, ocular epithelia and mucosae. Lesions heal with brown pigmentation but no scarring. Electron microscopy findings are distinct from those seen in the DM-EBS, with no evidence of tonofilament clumping.
    Defects in KRT5 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
    Defects in KRT5 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, althought it is less severe.
    Defects in KRT5 are the cause of epidermolysis bullosa simplex with mottled pigmentation (MP-EBS) [MIM:131960]. MP-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering at acral sites and 'mottled' pigmentation of the trunk and proximal extremities with hyper- and hypopigmentation macules.
    Defects in KRT5 are the cause of Dowling-Degos disease (DDD) [MIM:179850]; also known as Dowling-Degos-Kitamura disease or reticulate acropigmentation of Kitamura. DDD is an autosomal dominant genodermatosis. Affected individuals develop a postpubertal reticulate hyperpigmentation that is progressive and disfiguring, and small hyperkeratotic dark brown papules that affect mainly the flexures and great skin folds. Patients usually show no abnormalities of the hair or nails.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 58 kDa cytokeratin antibody
    • CK-5 antibody
    • CK5 antibody
    • Cytokeratin-5 antibody
    • Cytokeratin5 antibody
    • DDD antibody
    • DDD1 antibody
    • EBS2 antibody
    • epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody
    • K2C5_HUMAN antibody
    • K5 antibody
    • keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) antibody
    • Keratin 5 antibody
    • Keratin antibody
    • keratin complex 2, basic, gene 5 antibody
    • keratin, type II cytoskeletal 5 antibody
    • Keratin-5 antibody
    • Keratin5 antibody
    • KRT 5 antibody
    • Krt5 antibody
    • KRT5A antibody
    • type II cytoskeletal 5 antibody
    • Type-II keratin Kb5 antibody
    see all


  • ab17130 staining Cytokeratin 5 in Human keratinocytes by ICC (Immunocytochemistry). Cells were fixed with ethanol:acetone (1:1), permeabilized with Triton X 100 (0.1% for 5 minutes) and blocked with FCS/H2O2 3% (endo. PO) for 5 minutes at 21°C. Samples were incubated with primary antibody (1/100 in FBS Stain-Buffer) for 1 hour at 21°C. An undiluted HRP-conjugated Goat anti-rabbit/mouse IgG polyclonal was used as the secondary antibody. Negative cells were stained with Mayer's hematoxilin.

    See Abreview

  • ab171330 staining Cytokeratin 5 in human airway epithelial cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in formaldehyde, permeabilized using PBS/ 0.25% Triton for 10 minutes, blocked with 5% horse serum for 1 hour at room temperature and then incubated with ab17130 at a 1/200 dilution for 15 hours. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal used at a 1/750 dilution.

    See Abreview

  • Overlay histogram showing A431 cells stained with ab17130 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab17130, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


This product has been referenced in:
  • Hamilton NJI  et al. Using a Three-Dimensional Collagen Matrix to Deliver Respiratory Progenitor Cells to Decellularized Trachea In Vivo. Tissue Eng Part C Methods 25:93-102 (2019). Read more (PubMed: 30648458) »
  • Shrestha R  et al. BAP1 haploinsufficiency predicts a distinct immunogenic class of malignant peritoneal mesothelioma. Genome Med 11:8 (2019). Read more (PubMed: 30777124) »
See all 8 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC2 (pH6)
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C

Abcam user community

Verified customer

Submitted Feb 17 2017

Immunocytochemistry/ Immunofluorescence
Human Cell (airway epithelial cells)
airway epithelial cells
Yes - PBS triton 0.25% 10 min
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted May 17 2012

Human Cultured Cells (primary cells from human skin (keratinocytes / fib)
primary cells from human skin (keratinocytes / fib
EtOH:Acetone (1:1)
Yes - Triton-X-100 / 0,1% / 5 min
Blocking step
(agent) for 5 minute(s) · Concentration: 100% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jul 07 2010

Immunohistochemistry (Frozen sections)
Human Tissue sections (Human xenograft onto mouse. Section contains skin)
Human xenograft onto mouse. Section contains skin
Yes - 0.1% Triton X-100 in block and Ab staining solutions.
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 07 2009


Thank you for your enquiry. Those antibodies have been tested by different sources in different methods, and therefore currently are only guaranteed to work in the applications tested. It was reported to us that ab24647 worked in IHC as well as WB, unfortunately as we couldn't find out what type of IHC was tested we decided not to add this information to the datasheet. If you decide to go ahead and purchase one of those products, we encourage you to submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and make all product information available to researchers. If you send us an Abreview, we can offer you from 50 up to 300 Abcam points (Abpoints) based on submission time and type of application. The less characterised the product you review, the more Abpoints you will receive. Furthermore, you get 100 extra Abpoints for submitting an image. These Abpoints can then be claimed back as rewards. To find out more about our new Loyalty Scheme, Abpoints and rewards please visit our website at www.abcam.com/abreviews and look at Your Account panel (left bottom section on our home page). I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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