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    cytokeratin-6-antibody-ab150052.pdf

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Tags & Cell Markers Cell Type Markers Tumor Associated
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Anti-Cytokeratin 6 antibody (ab150052)

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Western blot - Anti-Cytokeratin 6 antibody (ab150052)

    Key features and details

    • Rabbit polyclonal to Cytokeratin 6
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-Cytokeratin 6 antibody
      See all Cytokeratin 6 primary antibodies
    • Description

      Rabbit polyclonal to Cytokeratin 6
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Chimpanzee, Gorilla
    • Immunogen

      Synthetic peptide corresponding to Human Cytokeratin 6 aa 1-100 conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab139537)

    • Positive control

      • This antibody gave a positive signal in A431 whole cell lysate.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Tags & Cell Markers
      • Cell Type Markers
      • Epi / Endo-thelial
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Intermediate Filaments
      • Class I
      • Cytokeratins

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    Our Abpromise guarantee covers the use of ab150052 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).

    Target

    • Tissue specificity

      Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.
    • Sequence similarities

      Belongs to the intermediate filament family.
    • Target information above from: UniProt accession P48668 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 286887 Human
      • Entrez Gene: 3853 Human
      • Entrez Gene: 3854 Human
      • Omim: 148041 Human
      • Omim: 148042 Human
      • Omim: 612315 Human
      • SwissProt: P02538 Human
      • SwissProt: P04259 Human
      • SwissProt: P48668 Human
      • Unigene: 700779 Human
      • Unigene: 709234 Human
      see all
    • Alternative names

      • CK 6A antibody
      • CK 6B antibody
      • CK 6C antibody
      • CK 6D antibody
      • CK 6E antibody
      • CK-6B antibody
      • CK-6C antibody
      • CK-6E antibody
      • Cytokeratin 6a antibody
      • Cytokeratin 6B antibody
      • Cytokeratin 6C antibody
      • Cytokeratin 6D antibody
      • Cytokeratin 6E antibody
      • Cytokeratin-6B antibody
      • Cytokeratin-6C antibody
      • Cytokeratin-6E antibody
      • K2C6C_HUMAN antibody
      • K6a keratin antibody
      • K6b keratin antibody
      • K6C antibody
      • K6c keratin antibody
      • K6d keratin antibody
      • K6e keratin antibody
      • Keratin antibody
      • Keratin K6h antibody
      • Keratin type II cytoskeletal 6A antibody
      • Keratin type II cytoskeletal 6B antibody
      • Keratin type II cytoskeletal 6C antibody
      • Keratin type II cytoskeletal 6D antibody
      • Keratin type II cytoskeletal 6E antibody
      • Keratin-6C antibody
      • KRT6A antibody
      • KRT6B antibody
      • KRT6C antibody
      • KRT6D antibody
      • KRT6E antibody
      • type II cytoskeletal 6C antibody
      • Type-II keratin Kb12 antibody
      see all

    Images

    • Western blot - Anti-Cytokeratin 6 antibody (ab150052)
      Western blot - Anti-Cytokeratin 6 antibody (ab150052)
      Anti-Cytokeratin 6 antibody (ab150052) at 1 µg/ml + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 60 kDa
      Observed band size: 60 kDa
      Additional bands at: 120 kDa, 35 kDa, 53 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab150052? Please let us know so that we can cite the reference in this datasheet.

    ab150052 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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