Anti-Cytokeratin 6 antibody (ab150052)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Cytokeratin 6 antibody
See all Cytokeratin 6 primary antibodies -
Description
Rabbit polyclonal to Cytokeratin 6 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla
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Immunogen
Synthetic peptide corresponding to Human Cytokeratin 6 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab139537) -
Positive control
- This antibody gave a positive signal in A431 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab150052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). |
Target
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Tissue specificity
Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 286887 Human
- Entrez Gene: 3853 Human
- Entrez Gene: 3854 Human
- Omim: 148041 Human
- Omim: 148042 Human
- Omim: 612315 Human
- SwissProt: P02538 Human
- SwissProt: P04259 Human
see all -
Alternative names
- CK 6A antibody
- CK 6B antibody
- CK 6C antibody
see all
Images
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Western blot - Anti-Cytokeratin 6 antibody (ab150052)Anti-Cytokeratin 6 antibody (ab150052) at 1 µg/ml + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Additional bands at: 120 kDa, 35 kDa, 53 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Protocols
Datasheets and documents
References
ab150052 has not yet been referenced specifically in any publications.
