Recombinant
RabMAb

Recombinant Anti-Cytokeratin 6 antibody [EPR1602Y] (Alexa Fluor® 488) (ab199068)

Overview

  • Product name

    Anti-Cytokeratin 6 antibody [EPR1602Y] (Alexa Fluor® 488)
    See all Cytokeratin 6 primary antibodies
  • Description

    Rabbit monoclonal [EPR1602Y] to Cytokeratin 6 (Alexa Fluor® 488)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cytokeratin 6 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HeLa cells Flow Cyt: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab199068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50.
ICC/IF 1/100.

Target

  • Tissue specificity

    Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Information by UniProt
  • Database links

  • Alternative names

    • CK 6A antibody
    • CK 6B antibody
    • CK 6C antibody
    • CK 6D antibody
    • CK 6E antibody
    • CK-6B antibody
    • CK-6C antibody
    • CK-6E antibody
    • Cytokeratin 6a antibody
    • Cytokeratin 6B antibody
    • Cytokeratin 6C antibody
    • Cytokeratin 6D antibody
    • Cytokeratin 6E antibody
    • Cytokeratin-6B antibody
    • Cytokeratin-6C antibody
    • Cytokeratin-6E antibody
    • K2C6C_HUMAN antibody
    • K6a keratin antibody
    • K6b keratin antibody
    • K6C antibody
    • K6c keratin antibody
    • K6d keratin antibody
    • K6e keratin antibody
    • Keratin antibody
    • Keratin K6h antibody
    • Keratin type II cytoskeletal 6A antibody
    • Keratin type II cytoskeletal 6B antibody
    • Keratin type II cytoskeletal 6C antibody
    • Keratin type II cytoskeletal 6D antibody
    • Keratin type II cytoskeletal 6E antibody
    • Keratin-6C antibody
    • KRT6A antibody
    • KRT6B antibody
    • KRT6C antibody
    • KRT6D antibody
    • KRT6E antibody
    • type II cytoskeletal 6C antibody
    • Type-II keratin Kb12 antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab199068 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab199068, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • ab199068 staining Cytokeratin 6 in Hela cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199068 at 1/100 dilution (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml. This was followed by an incubation at room temperature for 1h with ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed, at 1µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab199068 has not yet been referenced specifically in any publications.

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