Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1603Y] to Cytokeratin 6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Product nameAnti-Cytokeratin 6 antibody [EPR1603Y]
See all Cytokeratin 6 primary antibodies
DescriptionRabbit monoclonal [EPR1603Y] to Cytokeratin 6
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Cytokeratin 6 aa 550-650 (C terminal). P48668, P02538 and P04259.
Database link: P48668
- A431 cell lysate, human squamous cervical carcinoma, A431 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab52620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100000. Predicted molecular weight: 60 kDa.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
Tissue specificityConstitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.
Sequence similaritiesBelongs to the intermediate filament family.
- Information by UniProt
- CK 6A antibody
- CK 6B antibody
- CK 6C antibody
Ab52620 at 1/100-1/250 dilution staining human squamous cervical carcinoma; paraffin embedded.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/Immunofluorescence analysis of HaCaT (Human keratinocyte cell line) labelling Cytokeratin 6 with purified ab52620 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Anti-Cytokeratin 6 antibody [EPR1603Y] (ab52620) at 1/100000 dilution + A431 cell lysate at 10 µg
Goat anti-rabbit HRP antibody at 1/2000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Ab52620 at 1/100-1/250 dilution staining A431 cells.
ab52620 has been referenced in 2 publications.
- Khanom R et al. Keratin 17 Is Induced in Oral Cancer and Facilitates Tumor Growth. PLoS One 11:e0161163 (2016). PubMed: 27512993
- Szabo AZ et al. The CD44(+) ALDH(+) Population of Human Keratinocytes is Enriched for Epidermal Stem Cells with Long Term Repopulating Ability. Stem Cells : (2013). PubMed: 23335266