Recombinant Anti-Cytokeratin 6 antibody [EPR1603Y] (ab52620)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1603Y] to Cytokeratin 6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cytokeratin 6 antibody [EPR1603Y]
See all Cytokeratin 6 primary antibodies -
Description
Rabbit monoclonal [EPR1603Y] to Cytokeratin 6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Cytokeratin 6 aa 550-650 (C terminal). P48668, P02538 and P04259.
Database link: P48668 -
Positive control
- A431 cell lysate, human squamous cervical carcinoma, A431 cells. IHC-P: human normal skin tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1603Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100000. Predicted molecular weight: 60 kDa.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/100000. Predicted molecular weight: 60 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Tissue specificity
Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 286887 Human
- Entrez Gene: 3853 Human
- Entrez Gene: 3854 Human
- Omim: 148041 Human
- Omim: 148042 Human
- Omim: 612315 Human
- SwissProt: P02538 Human
- SwissProt: P04259 Human
see all -
Alternative names
- CK 6A antibody
- CK 6B antibody
- CK 6C antibody
see all
Images
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IHC image of Cytokeratin 6 staining in a section of formalin-fixed paraffin-embedded normal human skin* performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab52620, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre -
All lanes : Anti-Cytokeratin 6 antibody [EPR1603Y] (ab52620) at 1/1000 dilution
Lane 1 : N-GST tagged full-length recombinant human Cytokeratin 6A protein, 10 ng
Lane 2 : N-GST tagged full-length recombinant human Cytokeratin 5 protein, 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking buffer: 5% NFDM /TBST.
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Ab52620 at 1/100-1/250 dilution staining human squamous cervical carcinoma; paraffin embedded.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/Immunofluorescence analysis of HaCaT (Human keratinocyte cell line) labelling Cytokeratin 6 with purified ab52620 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
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Anti-Cytokeratin 6 antibody [EPR1603Y] (ab52620) at 1/100000 dilution + A431 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP antibody at 1/2000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa -
Ab52620 at 1/100-1/250 dilution staining A431 cells.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab52620 has been referenced in 2 publications.
- Khanom R et al. Keratin 17 Is Induced in Oral Cancer and Facilitates Tumor Growth. PLoS One 11:e0161163 (2016). PubMed: 27512993
- Szabo AZ et al. The CD44(+) ALDH(+) Population of Human Keratinocytes is Enriched for Epidermal Stem Cells with Long Term Repopulating Ability. Stem Cells : (2013). PubMed: 23335266