Overview

  • Product name
    Anti-Cytokeratin 6 antibody [Ks6.KA12]
    See all Cytokeratin 6 primary antibodies
  • Description
    Mouse monoclonal [Ks6.KA12] to Cytokeratin 6
  • Host species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, ICC, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A cytoskeletal preparation of human callus material was used for immunization.

Properties

Applications

Our Abpromise guarantee covers the use of ab18586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
IHC-P 1/10. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Target

  • Tissue specificity
    Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Information by UniProt
  • Database links
  • Alternative names
    • CK 6A antibody
    • CK 6B antibody
    • CK 6C antibody
    • CK 6D antibody
    • CK 6E antibody
    • CK-6C antibody
    • CK-6E antibody
    • Cytokeratin 6a antibody
    • Cytokeratin 6B antibody
    • Cytokeratin 6C antibody
    • Cytokeratin 6D antibody
    • Cytokeratin 6E antibody
    • Cytokeratin-6C antibody
    • Cytokeratin-6E antibody
    • K2C6C_HUMAN antibody
    • K6a keratin antibody
    • K6b keratin antibody
    • K6C antibody
    • K6c keratin antibody
    • K6d keratin antibody
    • K6e keratin antibody
    • Keratin antibody
    • Keratin K6h antibody
    • Keratin type II cytoskeletal 6A antibody
    • Keratin type II cytoskeletal 6B antibody
    • Keratin type II cytoskeletal 6C antibody
    • Keratin type II cytoskeletal 6D antibody
    • Keratin type II cytoskeletal 6E antibody
    • Keratin-6C antibody
    • KRT6A antibody
    • KRT6B antibody
    • KRT6C antibody
    • KRT6D antibody
    • KRT6E antibody
    • type II cytoskeletal 6C antibody
    • Type-II keratin Kb12 antibody
    see all

Images

  • ab18586 staining Cytokeratin 6 in human breast gland tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with methanol and blocked with 10% serum for 5 minutes. The sample was incubated with primary antibody (1/25) for 1 hour. An HRP-conjugated rabbit polyclonal to mouse IgG was used undiluted as secondary antibody. Ultravision ONE detection system has been used.

    See Abreview

  • ab18586 staining Cytokeratin 6 in breast organoids derived from normal human breast tissue by Immunocytochemistry.
    Cells were fixed in methanol and blocked using 10% serum for 5 minutes at 25°C. Samples were then incubated with ab18586 at a 1/25 for 1 hour at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal.

    See Abreview

  • Overlay histogram showing A431 cells stained with ab18586 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18586, 2µg/1x106 cells ) for 30 min at 22°C. The secondary antibody used was a goat anti-mous DyLight® 488 (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.

References

This product has been referenced in:
  • Wagner VP  et al. Histogenesis of keratoacanthoma: histochemical and immunohistochemical study. Oral Surg Oral Med Oral Pathol Oral Radiol 119:310-7 (2015). IHC-P ; Human . Read more (PubMed: 25488010) »
  • Holt BM  et al. Pig dorsum model for examining impaired wound healing at the skin-implant interface of percutaneous devices. J Mater Sci Mater Med 24:2181-93 (2013). Read more (PubMed: 23832453) »
See all 2 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer

Thank you for your enquiry. I have contacted the laboratory that provides the mouse monoclonal anti-cytokeratin 6 to us, ab18586. I am assuming they have data demonstrating mouse reactivity but I need to confirm this. If they do have data, then our guarantee will apply to mouse samples tested with one or more of the applications we list on the datasheet: flow cytometry, western blotting, immunocytochemistry, and immunohistochemistry.

We do not have small samples, but if you purchase an antibody and it fails for you, we will do our best to suggest a protocol modification that improves the result. If we are not able to help, and the application and species are listed on the datasheet as tested and reactive, then we will either provide a free replacement or refund the cost, within 6 months of purchase.

I will forward what I find out about ab18586 and mouse testing. Please let me know if you have other questions or concerns.

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Question
Answer

Thank you for your call today and for letting us know about the trouble with this antibody. I am sorry for the variability in the results, and I'm hoping that the replacement vial will work as expected.

The free of charge replacement vial will be arriving on the order ***. The expected delivery date has changed since we spoke, and it should arrive on Friday the 15th (one week from today) due to the transfer from our UK office. I hope this will not be too much of a problem, but please let me know if so.

Please keep me updated about the results, and let me know if you have any further questions or if there is anything else that we can do fory ou.

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Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Epithelial)
Specification
Epithelial
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Mr. Daniel Holod-Betz

Verified customer

Submitted May 25 2012

Question
Answer

Thank you for contacting Abcam. The antibody ab18586 recognizes all 6 isoforms of keratin. I have searched our catalogue to find a antibody specific to CK6b, however I have not been able to find one. I would recommend searching www.biocompare.com to see if you can find an antibody there. If there is anything else I can help you with, please let me kn

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (Primary breast)
Specification
Primary breast
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C

Dr. Rene Villadsen

Verified customer

Submitted Jun 27 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Breast gland)
Specification
Breast gland
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10%

Dr. Rene Villadsen

Verified customer

Submitted Sep 25 2009

Answer

Thank you for your enquiry. I have received a reply from the originators of ab18586 and ab25820. The concentration ab18586 is 5mg/ml and the concentration of ab25820 is 2ug/ml. Please don't hesitate to contact us again if you have further questions.

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Question
Answer

Thank you for your enquiry. To our knowledge, these antibodies have not yet been tested for cross-reactivity with nectin 1. If we receive future information regarding specificity, we will add such information to the online datasheets. If you have any additional questions, please do contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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